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Correction published on 19 February 2019, see Molecules 2019, 24(4), 745.
Article

Anti-Inflammatory Effects of Aurantio-Obtusin from Seed of Cassia obtusifolia L. through Modulation of the NF-κB Pathway

by 1,2, 1,2, 1,2, 1,2, 1,2, 1,2,*, 1,2,* and 1,2
1
Research Center of Traditional Chinese Medicine Information Engineering, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
2
Beijing Key Laboratory of Traditional Chinese Medicine Basics and New Drug Research, Research Center of Traditional Chinese Medicine Information Engineering, Beijing University of Chinese Medicine, Beijing 100102, China
*
Authors to whom correspondence should be addressed.
Molecules 2018, 23(12), 3093; https://doi.org/10.3390/molecules23123093
Received: 5 November 2018 / Revised: 18 November 2018 / Accepted: 21 November 2018 / Published: 27 November 2018
Aurantio-obtusin, an anthraquinone compound, isolated from dried seeds of Cassia obtusifolia L. (syn. Senna obtusifolia; Fabaceae) and Cassia tora L. (syn. Senna tora). Although the biological activities of Semen Cassiae have been reported, the anti-inflammatory mechanism of aurantio-obtusin, its main compound, on RAW264.7 cells, remained unknown. We investigated the anti-inflammatory effect of aurantio-obtusin on lipopolysaccharide- (LPS)-induced RAW264.7 cells in vitro and elucidated the possible underlying molecular mechanisms. Nitric oxide production (NO) and prostaglandin E2 (PGE2) were measured by the Griess colorimetric method and enzyme-linked immunosorbent assay (ELISA), respectively. Protein expression levels of cyclooxygenase 2 (COX-2) were monitored by cell-based ELISA. Interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-α) synthesis were analyzed using ELISA. The mRNA expression of nitric oxide synthase (iNOS), COX-2, and the critical pro-inflammatory cytokines (IL-6 and TNF-α) were detected by quantitative real-time PCR. Aurantio-obtusin significantly decreased the production of NO, PGE2, and inhibited the protein expression of COX-2, TNF-α and IL-6, which were similar to those gene expression of iNOS, COX-2, TNF-α and IL-6 (p < 0.01). Consistent with the pro-inflammatory gene expression, the Aurantio-obtusin efficiently reduced the LPS-induced activation of nuclear factor-κB in RAW264.7 cells. These results suggested that aurantio-obtusin may function as a therapeutic agent and can be considered in the further development of treatments for a variety of inflammatory diseases. Further studies may provide scientific evidence for the use of aurantio-obstusin as a new therapeutic agent for inflammation-related diseases. View Full-Text
Keywords: inflammation; NF-κB; Semen Cassiae; anthraquinone inflammation; NF-κB; Semen Cassiae; anthraquinone
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MDPI and ACS Style

Hou, J.; Gu, Y.; Zhao, S.; Huo, M.; Wang, S.; Zhang, Y.; Qiao, Y.; Li, X. Anti-Inflammatory Effects of Aurantio-Obtusin from Seed of Cassia obtusifolia L. through Modulation of the NF-κB Pathway. Molecules 2018, 23, 3093. https://doi.org/10.3390/molecules23123093

AMA Style

Hou J, Gu Y, Zhao S, Huo M, Wang S, Zhang Y, Qiao Y, Li X. Anti-Inflammatory Effects of Aurantio-Obtusin from Seed of Cassia obtusifolia L. through Modulation of the NF-κB Pathway. Molecules. 2018; 23(12):3093. https://doi.org/10.3390/molecules23123093

Chicago/Turabian Style

Hou, Jingyi; Gu, Yu; Zhao, Shuai; Huo, Mengqi; Wang, Shifeng; Zhang, Yanling; Qiao, Yanjiang; Li, Xi. 2018. "Anti-Inflammatory Effects of Aurantio-Obtusin from Seed of Cassia obtusifolia L. through Modulation of the NF-κB Pathway" Molecules 23, no. 12: 3093. https://doi.org/10.3390/molecules23123093

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