The Effect of Pressure and Solvent on the Supercritical Fluid Chromatography Separation of Tocol Analogs in Palm Oil
Engineering and Processing Research Division, Malaysian Palm Oil Board, 6, Persiaran Institusi, Bandar Baru Bangi, Kajang 43000, Selangor, Malaysia
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Molecules 2017, 22(9), 1424; https://doi.org/10.3390/molecules22091424
Received: 21 June 2017 / Revised: 15 August 2017 / Accepted: 16 August 2017 / Published: 29 August 2017
(This article belongs to the Special Issue Sub- and Supercritical Fluids and Green Chemistry)
There are six tocol analogs present in palm oil, namely α-tocopherol (α-T), α-tocomonoenol (α-T1), α-tocotrienol (α-T3), γ-tocotrienol (γ-T3), β-tocotrioenol (β-T3) and δ-tocotrienol (δ-T3). These analogs were difficult to separate chromatographically due to their similar structures, physical and chemical properties. This paper reports on the effect of pressure and injection solvent on the separation of the tocol analogs in palm oil. Supercritical CO2 modified with ethanol was used as the mobile phase. Both total elution time and resolution of the tocol analogs decreased with increased pressure. Ethanol as an injection solvent resulted in peak broadening of the analogs within the entire pressure range studied. Solvents with an eluent strength of 3.4 or less were more suitable for use as injecting solvents.
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Keywords:
palm; supercritical fluid chromatography; tocol analogs; tocotrienol
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MDPI and ACS Style
Ng, M.H.; Kushairi, A. The Effect of Pressure and Solvent on the Supercritical Fluid Chromatography Separation of Tocol Analogs in Palm Oil. Molecules 2017, 22, 1424. https://doi.org/10.3390/molecules22091424
AMA Style
Ng MH, Kushairi A. The Effect of Pressure and Solvent on the Supercritical Fluid Chromatography Separation of Tocol Analogs in Palm Oil. Molecules. 2017; 22(9):1424. https://doi.org/10.3390/molecules22091424
Chicago/Turabian StyleNg, Mei H.; Kushairi, Ahmad. 2017. "The Effect of Pressure and Solvent on the Supercritical Fluid Chromatography Separation of Tocol Analogs in Palm Oil" Molecules 22, no. 9: 1424. https://doi.org/10.3390/molecules22091424
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