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Molecules 2017, 22(8), 1347;

Application of Solution NMR to Structural Studies on α-Helical Integral Membrane Proteins

Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungbuk 27478, Korea
Department of Biochemistry, Vanderbilt University, Nashville, TN 37204, USA
Department of Molecular Science and Technology & College of Pharmacy, Ajou University, Suwon 16499, Korea
The Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Gwanak-gu, Seoul 151-742, Korea
College of Pharmacy, Chungbuk National University, Cheongju 28160, Chungbuk, Korea
These authors contributed equally to this paper.
Authors to whom correspondence should be addressed.
Received: 31 July 2017 / Revised: 10 August 2017 / Accepted: 12 August 2017 / Published: 15 August 2017
(This article belongs to the Special Issue Recent Advances in Biomolecular NMR Spectroscopy)
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A large portion of proteins in living organisms are membrane proteins which play critical roles in the biology of the cell, from maintenance of the biological membrane integrity to communication of cells with their surroundings. To understand their mechanism of action, structural information is essential. Nevertheless, structure determination of transmembrane proteins is still a challenging area, even though recently the number of deposited structures of membrane proteins in the PDB has rapidly increased thanks to the efforts using X-ray crystallography, electron microscopy, and solid and solution nuclear magnetic resonance (NMR) technology. Among these technologies, solution NMR is a powerful tool for studying protein-protein, protein-ligand interactions and protein dynamics at a wide range of time scales as well as structure determination of membrane proteins. This review provides general and useful guideline for membrane protein sample preparation and the choice of membrane-mimetic media, which are the key step for successful structural analysis. Furthermore, this review provides an opportunity to look at recent applications of solution NMR to structural studies on α-helical membrane proteins through some success stories. View Full-Text
Keywords: solution NMR; α-helical integral membrane protein; structure; membrane-mimetics; isotope labeling solution NMR; α-helical integral membrane protein; structure; membrane-mimetics; isotope labeling

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Sim, D.-W.; Lu, Z.; Won, H.-S.; Lee, S.-N.; Seo, M.-D.; Lee, B.-J.; Kim, J.-H. Application of Solution NMR to Structural Studies on α-Helical Integral Membrane Proteins. Molecules 2017, 22, 1347.

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