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Molecules 2017, 22(8), 1291;

Development of HA/Ag-NPs Composite Coating from Green Process for Hip Applications

Centro de Investigación en Materiales Avanzados (CIMAV), Miguel de Cervantes 120, Chihuahua 31109, Mexico
Departamento de Biotecnologia, Universidade de Sorocaba (UNISO), Rod. Raposo Tavares, km 92.5, Sorocaba 18023-000, Brazil
Departamento de Engenharia Ambiental, Universidade Estadual Paulista (UNESP), Av. Três de Março 511, Sorocaba 18087-180, Brazil
Centro de Investigación en Química Aplicada (CIQA), Blvd. Enrique Reyna 140, Saltillo 25250, Mexico
Centro de Capacitación, Investigación, Innovación y Transferencia Tecnológica, Universidad Tecnológica de Chihuahua Sur (UTCh Sur), Km. 3 Carretera Chihuahua-Aldama, Chihuahua 31313, Mexico
Author to whom correspondence should be addressed.
Received: 9 June 2017 / Accepted: 31 July 2017 / Published: 8 August 2017
(This article belongs to the Special Issue Antibacterial Materials and Coatings)
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In the present study, biological hydroxyapatite (HA) was obtained from bovine bones through a thermal process. A total of 0% and 1% of silver nanoparticles (Ag-NPs) synthesized from Opuntia ficus (nopal) were added to the biological hydroxyapatite coatings using an atmospheric plasma spray (APS) on a Ti6Al4V substrate. Following this, its antimicrobial efficiency was evaluated against the following bacterial strains: Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. This was conducted according to the Japanese Industrial Standard (JIS) Z2801:2000 “Antimicrobial Product-Test for Antimicrobial Activity and Efficacy”. Scanning electron microscopy (SEM) showed that the silver nanoparticles (Ag-NPs) were evenly distributed on the coating surface. Energy dispersive X-ray spectroscopy (EDX) shows that apatite deposition occurs on a daily basis, maintaining a Ca/P rate between 2.12 and 1.45. Biocompatibility properties were evaluated with osteoblast-like cells (MC3T3-E1) by single-cell gel electrophoresis assay and Tali image cytometry. View Full-Text
Keywords: biological hydroxyapatite; coating; silver nanoparticles; atmospheric plasma spray; antibacterial test; cytotoxic test biological hydroxyapatite; coating; silver nanoparticles; atmospheric plasma spray; antibacterial test; cytotoxic test

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Lozoya-Rodríguez, D.A.; De Lima, R.; Fraceto, L.F.; Ledezma Pérez, A.; Bazaldua Domínguez, M.; Gómez Batres, R.; Reyes Rojas, A.; Orozco Carmona, V. Development of HA/Ag-NPs Composite Coating from Green Process for Hip Applications. Molecules 2017, 22, 1291.

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