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Molecules 2017, 22(7), 1226;

Study on Chemical Profile and Neuroprotective Activity of Myrica rubra Leaf Extract

Department of Pharmacy, Xiamen Medical College, Xiamen 361023, China
Engineering Research Center of Marine Biological Resource Comprehensive Utilization, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
Fineboon Dairy Nutrition Institute, Shaanxi Dairy Co. Ltd., Xianyang 712000, China
Department of Food Science, National Taiwan Ocean University, Keelung 202, Taiwan
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
Xiamen Key Laboratory of Marine Medicinal Natural Products and Cell Engineering, Xiamen Medical College, Xiamen 361008, China
Key Laboratory for Dao-Di Herbs Biotechnology of Fujian Province, Xiamen Medical College, Xiamen 361023, China
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Received: 7 June 2017 / Revised: 18 July 2017 / Accepted: 18 July 2017 / Published: 24 July 2017
(This article belongs to the Section Natural Products Chemistry)
Full-Text   |   PDF [2191 KB, uploaded 26 July 2017]   |  


The chemical profile of Myrica rubra (a native species in China) leaf extract was investigated by UPLC-PDA-HRMS, and the neuroprotective activity of two characteristic constituents, myricanol and myricetrin, was evaluated with N2a cells using H2O2-inducedoxidative challenge through a series of methods, e.g., MTT assay, ROS assay and [Ca2+]i assay. Among the 188 constituents detected in the extract of Myrica rubra leaf, 116 were identified definitely or tentatively by the comprehensive utilization of precise molecular weight and abundant multistage fragmentation information obtained by quadrupole orbitrap mass spectrometry. In addition, 14 potential new compounds were reported for the first time. This work established an example for the research of microconstituents in a complex analyte and revealed that suppression of H2O2-induced cytotoxicity in N2a cells was achieved by the pretreatment with myricanol. The evidence suggested myricanol may potentially serve as a remedy for prevention and therapy of neurodegenerative diseases induced by oxidative stress. View Full-Text
Keywords: UPLC-PDA-HRMS; Myrica rubra; neuroprotective activity UPLC-PDA-HRMS; Myrica rubra; neuroprotective activity

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Chen, P.; Lin, X.; Yang, C.-H.; Tang, X.; Chang, Y.-W.; Zheng, W.; Luo, L.; Xu, C.; Chen, Y.-H. Study on Chemical Profile and Neuroprotective Activity of Myrica rubra Leaf Extract. Molecules 2017, 22, 1226.

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