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Open AccessArticle

Metabolomics Characterization of Two Apocynaceae Plants, Catharanthus roseus and Vinca minor, Using GC-MS and LC-MS Methods in Combination

by 1, 1, 2, 1,* and 2,*
1
Center for Ecological Research, Northeast Forestry University, Harbin 150040, China
2
Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Molecules 2017, 22(6), 997; https://doi.org/10.3390/molecules22060997
Received: 12 May 2017 / Revised: 12 June 2017 / Accepted: 13 June 2017 / Published: 17 June 2017
Catharanthus roseus (C. roseus) and Vinca minor (V. minor) are two common important medical plants belonging to the family Apocynaceae. In this study, we used non-targeted GC-MS and targeted LC-MS metabolomics to dissect the metabolic profile of two plants with comparable phenotypic and metabolic differences. A total of 58 significantly different metabolites were present in different quantities according to PCA and PLS-DA score plots of the GC-MS analysis. The 58 identified compounds comprised 16 sugars, eight amino acids, nine alcohols and 18 organic acids. We subjected these metabolites into KEGG pathway enrichment analysis and highlighted 27 metabolic pathways, concentrated on the TCA cycle, glycometabolism, oligosaccharides, and polyol and lipid transporter (RFOS). Among the primary metabolites, trehalose, raffinose, digalacturonic acid and gallic acid were revealed to be the most significant marker compounds between the two plants, presumably contributing to species-specific phenotypic and metabolic discrepancy. The profiling of nine typical alkaloids in both plants using LC-MS method highlighted higher levels of crucial terpenoid indole alkaloid (TIA) intermediates of loganin, serpentine, and tabersonine in V. minor than in C. roseus. The possible underlying process of the metabolic flux from primary metabolism pathways to TIA synthesis was discussed and proposed. Generally speaking, this work provides a full-scale comparison of primary and secondary metabolites between two medical plants and a metabolic explanation of their TIA accumulation and phenotype differences. View Full-Text
Keywords: Catharanthus roseus; Vinca minor; GC-MS; LC-MS; metabolomics; TIAs Catharanthus roseus; Vinca minor; GC-MS; LC-MS; metabolomics; TIAs
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MDPI and ACS Style

Chen, Q.; Lu, X.; Guo, X.; Guo, Q.; Li, D. Metabolomics Characterization of Two Apocynaceae Plants, Catharanthus roseus and Vinca minor, Using GC-MS and LC-MS Methods in Combination. Molecules 2017, 22, 997. https://doi.org/10.3390/molecules22060997

AMA Style

Chen Q, Lu X, Guo X, Guo Q, Li D. Metabolomics Characterization of Two Apocynaceae Plants, Catharanthus roseus and Vinca minor, Using GC-MS and LC-MS Methods in Combination. Molecules. 2017; 22(6):997. https://doi.org/10.3390/molecules22060997

Chicago/Turabian Style

Chen, Qi; Lu, Xueyan; Guo, Xiaorui; Guo, Qingxi; Li, Dewen. 2017. "Metabolomics Characterization of Two Apocynaceae Plants, Catharanthus roseus and Vinca minor, Using GC-MS and LC-MS Methods in Combination" Molecules 22, no. 6: 997. https://doi.org/10.3390/molecules22060997

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