Synthesis of Aminoglycoside-2′-O-Methyl Oligoribonucleotide Fusions
1
Department of Chemistry, University of Turku, Vatselankatu 2, 20014 Turku, Finland
2
Centre of New Technologies, University of Warsaw, Banacha 2c, 02 097 Warsaw, Poland
3
College of Inter-Faculty Individual Studies in Mathematics and Natural Sciences, University of Warsaw, Banacha 2c, 02 097 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Molecules 2017, 22(5), 760; https://doi.org/10.3390/molecules22050760
Received: 24 April 2017 / Revised: 4 May 2017 / Accepted: 6 May 2017 / Published: 8 May 2017
(This article belongs to the Special Issue Synthesis and Applications of Oligonucleotide Conjugates)
Abstract
Phosphoramidite building blocks of ribostamycin (3 and 4), that may be incorporated at any position of the oligonucleotide sequence, were synthesized. The building blocks, together with a previously described neomycin-modified solid support, were applied for the preparation of aminoglycoside-2′-O-methyl oligoribonucleotide fusions. The fusions were used to clamp a single strand DNA sequence (a purine-rich strand of c-Myc promoter 1) to form triple helical 2′-O-methyl RNA/DNA-hybrid constructs. The potential of the aminoglycoside moieties to stabilize the triple helical constructs were studied by UV-melting profile analysis. View Full-TextKeywords:
aminoglycosides; triple helices; oligonucleotide conjugates
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Granqvist, L.; Kraszewski, A.; Tähtinen, V.; Virta, P. Synthesis of Aminoglycoside-2′-O-Methyl Oligoribonucleotide Fusions. Molecules 2017, 22, 760.
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