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Molecules 2017, 22(12), 2195; https://doi.org/10.3390/molecules22122195

Effect of Oxylipins, Terpenoid Precursors and Wounding on Soft Corals’ Secondary Metabolism as Analyzed via UPLC/MS and Chemometrics

1
Department of Pharmacognosy, College of Pharmacy, Cairo University, Kasr El Aini St., Cairo 11562, Egypt
2
Department of Chemistry, School of Sciences & Engineering, The American University in Cairo, New Cairo 11835, Egypt
3
Leibniz Centre for Tropical Marine Research, Fahrenheit Str.6, D-28359 Bremen, Germany
4
Department of Geosciences, Bremen University, Fahrenheit Str.6, D-28359 Bremen, Germany
5
Department of Pharmacognosy, College of Pharmacy, Modern Science and Arts University, Cairo 12566, Egypt
6
Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle, Germany
*
Author to whom correspondence should be addressed.
Received: 28 October 2017 / Revised: 30 November 2017 / Accepted: 2 December 2017 / Published: 10 December 2017
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Abstract

The effect of three oxylipin analogues, a terpenoid intermediate and wounding on the secondary metabolism of the soft corals Sarcophyton glaucum and Lobophyton pauciflorum was assessed. Examined oxylipins included prostaglandin (PG-E1), methyl jasmonate (MeJA), and arachidonic acid (AA) in addition to the diterpene precursor geranylgeranylpyrophosphate (GGP). Post-elicitation, metabolites were extracted from coral heads and analyzed via UPLC-MS followed by multivariate data analyses. Both supervised and unsupervised data analyses were used for sample classification. Multivariate data analysis revealed clear segregation of PG-E1 and MeJA elicited S. glaucum at 24 and 48 h post elicitation from other elicitor samples and unelicited control group. PG-E1 was found more effective in upregulating S. glaucum terpene/sterol levels compared to MeJA. Metabolites showing upregulation in S. glaucum include campestene-triol and a cembranoid, detected at ca. 30- and 2-fold higher levels compared to unelicited corals. Such an elicitation effect was less notable in the other coral species L. pauciflorum, suggesting a differential oxylipin response in soft corals. Compared to MeJA and PG, no elicitation effect was observed for GGP, AA or wounding on the metabolism of either coral species. View Full-Text
Keywords: oxylipins; soft corals; cembranoids; chemometrics; sterols oxylipins; soft corals; cembranoids; chemometrics; sterols
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Farag, M.A.; Westphal, H.; Eissa, T.F.; Wessjohann, L.A.; Meyer, A. Effect of Oxylipins, Terpenoid Precursors and Wounding on Soft Corals’ Secondary Metabolism as Analyzed via UPLC/MS and Chemometrics. Molecules 2017, 22, 2195.

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