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Open AccessArticle

Preparation of β-CD-Ellagic Acid Microspheres and Their Effects on HepG2 Cell Proliferation

1
College of Life Science, Zaozhuang University, Zaozhuang 277160, China
2
College of Medical Science, Zaozhuang Vocational College, Zaozhuang 277800, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Molecules 2017, 22(12), 2175; https://doi.org/10.3390/molecules22122175
Received: 13 October 2017 / Revised: 29 November 2017 / Accepted: 6 December 2017 / Published: 8 December 2017
(This article belongs to the Section Medicinal Chemistry)
Objective: In this study, β-cyclodextrin (β-CD) was chosen as the coating for ellagic acid to prepare ellagic acid microspheres, and the effect of microspheres on the growth of HepG2 cells was observed. Methods: Scanning electron microscopy, infrared spectroscopy, and release rate analysis were used to identify the formation of ellagic acid microspheres. Methyl thiazolyl tetrazolium (MTT) assay was used to detect the effect of different concentrations of ellagic acid microspheres on tumor cell proliferation at 6, 12, 24 and 36 h, and cell morphology and quantity were observed using hematoxylin-eosin (HE) staining. Single-cell gel electrophoresis was used to observe the effect of ellagic acid microspheres on the DNA damage of HepG2 cells, and the Olive tail moment and the mRNA expression of tumor suppressor protein gene p53 was measured. Results: β-CD could be used as wrapping material of ellagic acid to prepare ellagic acid microspheres. HepG2 cell proliferation could be inhibited by 0.1, 0.3 and 0.5 g/L of ellagic acid microspheres in a dose- and time-dependent manner, and the mechanism of proliferation inhibition was related to DNA damage and cell apoptosis. Conclusion: Preparing ellagic acid microspheres with β-CD is feasible, and ellagic acid microspheres have potential therapeutic value (anticancer). View Full-Text
Keywords: β-CD-ellagic acid microspheres; preparation; identification; HepG2 cell proliferations β-CD-ellagic acid microspheres; preparation; identification; HepG2 cell proliferations
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MDPI and ACS Style

Wang, H.; Zhang, Y.; Tian, Z.; Ma, J.; Kang, M.; Ding, C.; Ming, D. Preparation of β-CD-Ellagic Acid Microspheres and Their Effects on HepG2 Cell Proliferation. Molecules 2017, 22, 2175.

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