The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells
AbstractMelatonin (MLT) plays an important role in regulating the physiological cycle of seasonal breeding animals. Melatonin receptor I (MT1) is effectively expressed in the cambium layer of deer antler. However, the function and metabolic mechanism of MLT/MT1 signaling in the mesenchymal cells of sika deer remain to be further elucidated. In this work, we detected the effects of MLT/MT1 signaling on mesenchymal cells proliferation and the interaction between MLT/MT1 and IGF1/IGF1-R signaling. The results show that (1) deer antler mesenchymal cells actually express MT1; (2) exogenous melatonin significantly promotes mesenchymal cells proliferation, while MT1 knock-down significantly impairs the positive effects of melatonin; and (3) melatonin significantly enhanced IGF1/IGF1-R signaling, as both the expression of IGF1 and IGF-1R increased, while MT1 knock-down significantly decreased IGF1-R expression and IGF1 synthesis. In summary, these data verified that MLT/MT1 signaling plays a crucial role in antler mesenchymal proliferation, which may be mediated by IGF1/IGF1-R. View Full-Text
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Yang, F.; He, C.; Sun, X.; Wang, J.; Luo, C.; Liu, G.; Yang, L.; Xiong, J.; Huo, L. The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells. Molecules 2017, 22, 1793.
Yang F, He C, Sun X, Wang J, Luo C, Liu G, Yang L, Xiong J, Huo L. The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells. Molecules. 2017; 22(10):1793.Chicago/Turabian Style
Yang, Feifei; He, Changjiu; Sun, Xuyang; Wang, Jing; Luo, Can; Liu, Guoshi; Yang, Liguo; Xiong, Jiajun; Huo, Lijun. 2017. "The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells." Molecules 22, no. 10: 1793.
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