Rate of Homologous Desensitization and Internalization of the GLP-1 Receptor
Abstract
:1. Introduction
2. Results
2.1. Validation of N-Terminally Labelled GLP-1R
2.2. Homologous Desensitization of GLP-1R
2.3. Internalization of GLP-1R
2.4. Visualization of Internalized GLP-1R
3. Discussion
4. Materials and Methods
4.1. Construction of cDNA
4.2. Cell Culture and Transfection of Cells
4.3. Luciferase Assay
4.4. Desensitization Assay
4.5. Internalization Assay
4.6. Confocal Microscopy
4.7. Data Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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- Sample Availability: Samples of the receptor constructs are available from the authors.
Control (Media) ↓ | Control (Media) ↓ | Pre-Stimulate with 1 µM GLP-1 (5–120) min ↓ | Pre-Stimulate with 1 µM GLP-1 (5–120) min ↓ |
Wash ↓ | Wash ↓ | Wash ↓ | Wash ↓ |
Stimulate 1 µM GLP-1 15 min (Stimulated) ↓ | Media (Non-Stimulated) ↓ | Stimulate 1 µM GLP-1 15 min (Stimulated) ↓ | Media (Non-Stimulated) ↓ |
Assay | Assay | Assay | Assay |
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Shaaban, G.; Oriowo, M.; Al-Sabah, S. Rate of Homologous Desensitization and Internalization of the GLP-1 Receptor. Molecules 2017, 22, 22. https://doi.org/10.3390/molecules22010022
Shaaban G, Oriowo M, Al-Sabah S. Rate of Homologous Desensitization and Internalization of the GLP-1 Receptor. Molecules. 2017; 22(1):22. https://doi.org/10.3390/molecules22010022
Chicago/Turabian StyleShaaban, Ghina, Mabayoje Oriowo, and Suleiman Al-Sabah. 2017. "Rate of Homologous Desensitization and Internalization of the GLP-1 Receptor" Molecules 22, no. 1: 22. https://doi.org/10.3390/molecules22010022
APA StyleShaaban, G., Oriowo, M., & Al-Sabah, S. (2017). Rate of Homologous Desensitization and Internalization of the GLP-1 Receptor. Molecules, 22(1), 22. https://doi.org/10.3390/molecules22010022