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Molecules 2016, 21(12), 1619;

A New Two-Photon Ratiometric Fluorescent Probe for Detecting Alkaline Phosphatase in Living Cells

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
Environment Monitoring Department, Changsha Environmental Protection College, Changsha 410004, China
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 19 October 2016 / Revised: 20 November 2016 / Accepted: 22 November 2016 / Published: 25 November 2016
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Alkaline phosphatase (ALP) is an important diagnostic indicator of many human diseases. To quantitatively track ALP in biosystems, herein, for the first time, we report an efficient two-photon ratiometric fluorescent probe, termed probe 1 and based on classic naphthalene derivatives with a donor–π–acceptor (D–π–A) structure and deprotection of the phosphoric acid moiety by ALP. The presence of ALP causes the cleave of the phosphate group from naphthalene derivatives and the phosphate group changes the ability of the intramolecular charge transfer (ICT) and remarkably alters the probe’s photophysical properties, thus an obvious ratiometric signal with an isoemissive point is observed. The fluorescence intensity ratio displayed a linear relationship against the concentration of ALP in the concentration range from 20 to 180 U/L with the limit of detection of 2.3 U/L. Additionally, the probe 1 is further used for fluorescence imaging of ALP in living cells under one-photon excitation (405 nm) or two-photon excitation (720 nm), which showed a high resolution imaging, thus demonstrating its practical application in biological systems. View Full-Text
Keywords: two-photon; ratiometric; alkaline phosphatase (ALP); intramolecular charge transfer (ICT) two-photon; ratiometric; alkaline phosphatase (ALP); intramolecular charge transfer (ICT)

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Zhou, X.; Jiang, Y.; Zhao, X.; Zhu, Y. A New Two-Photon Ratiometric Fluorescent Probe for Detecting Alkaline Phosphatase in Living Cells. Molecules 2016, 21, 1619.

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