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Molecules 2016, 21(11), 1459;

Phylogenetic and Diversity Analysis of Dactylis glomerata Subspecies Using SSR and IT-ISJ Markers

Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, China
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 31 August 2016 / Revised: 13 October 2016 / Accepted: 26 October 2016 / Published: 31 October 2016
(This article belongs to the Section Molecular Diversity)
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The genus Dactylis, an important forage crop, has a wide geographical distribution in temperate regions. While this genus is thought to include a single species, Dactylis glomerata, this species encompasses many subspecies whose relationships have not been fully characterized. In this study, the genetic diversity and phylogenetic relationships of nine representative Dactylis subspecies were examined using SSR and IT-ISJ markers. In total, 21 pairs of SSR primers and 15 pairs of IT-ISJ primers were used to amplify 295 polymorphic bands with polymorphic rates of 100%. The average polymorphic information contents (PICs) of SSR and IT-ISJ markers were 0.909 and 0.780, respectively. The combined data of the two markers indicated a high level of genetic diversity among the nine D. glomerata subspecies, with a Nei’s gene diversity index value of 0.283 and Shannon’s diversity of 0.448. Preliminarily phylogenetic analysis results revealed that the 20 accessions could be divided into three groups (A, B, C). Furthermore, they could be divided into five clusters, which is similar to the structure analysis with K = 5. Phylogenetic placement in these three groups may be related to the distribution ranges and the climate types of the subspecies in each group. Group A contained eight accessions of four subspecies, originating from the west Mediterranean, while Group B contained seven accessions of three subspecies, originating from the east Mediterranean. View Full-Text
Keywords: Dactylis; subspecies; SSR; IT-ISJ; genetic diversity; phylogenetic analysis Dactylis; subspecies; SSR; IT-ISJ; genetic diversity; phylogenetic analysis

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Yan, D.; Zhao, X.; Cheng, Y.; Ma, X.; Huang, L.; Zhang, X. Phylogenetic and Diversity Analysis of Dactylis glomerata Subspecies Using SSR and IT-ISJ Markers. Molecules 2016, 21, 1459.

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