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Molecules 2016, 21(10), 1411;

Potential Anticancer Effects of Polyphenols from Chestnut Shell Extracts: Modulation of Cell Growth, and Cytokinomic and Metabolomic Profiles

CROM, Istituto Nazionale Tumori “Fondazione G. Pascale”—IRCCS, Napoli 80131, Italy
Consiglio Nazionale delle Ricerche, Istituto di Scienze dell’Alimentazione, Via Roma 64, Avellino 83100, Italy
Direttore Scientifico, IRCCS Istituto Nazionale Tumori “Fondazione G. Pascale”, Napoli 80131, Italy
Dipartimento di Scienze e Tecnologie, Università degli Studi del Sannio, Via Port’Arsa 11, Benevento 82100, Italy
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 20 September 2016 / Revised: 18 October 2016 / Accepted: 18 October 2016 / Published: 21 October 2016
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In this study, a hydroalcoholic chestnut shell extract was characterized and tested on six different human cell lines. Gallic, ellagic, and syringic acids were the most abundant non-condensed compounds in the chestnut extract, as determined by high performance liquid chromatography (HPLC). Tannins were mainly represented by condensed monomeric units of epigallocatechin and catechin/epicatechin. After 48 h of treatment, only the human hepatoblastoma HepG2 cells reached an inhibition corresponding to IC50 with an increase of apoptosis and mitochondrial depolarization. The cytokinome evaluation before and after treatment revealed that the vascular endothelial growth factor (VEGF) and the tumor necrosis factor (TNF)-α decreased after the treatment, suggesting a potential anti-angiogenic and anti-inflammatory effect of this extract. Moreover, the metabolome evaluation by 1H-NMR evidenced that the polyphenols extracted from chestnut shell (PECS) treatment affected the levels of some amino acids and other metabolites. Overall, these data highlight the effects of biomolecules on cell proliferation, apoptosis, cell cycle and mitochondrial depolarization, and on cytokinomics and metabolomics profiles. View Full-Text
Keywords: chestnut shells extract; polyphenols; cancer chestnut shells extract; polyphenols; cancer

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Sorice, A.; Siano, F.; Capone, F.; Guerriero, E.; Picariello, G.; Budillon, A.; Ciliberto, G.; Paolucci, M.; Costantini, S.; Volpe, M.G. Potential Anticancer Effects of Polyphenols from Chestnut Shell Extracts: Modulation of Cell Growth, and Cytokinomic and Metabolomic Profiles. Molecules 2016, 21, 1411.

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