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Molecules 2016, 21(1), 35;

A Herbal Formula, Atofreellage, Ameliorates Atopic Dermatitis-Like Skin Lesions in an NC/Nga Mouse Model

Liver and Immunology Research-Center, Daejeon Oriental Hospital of Daejeon University, 176-9, Daeheung-ro, Jung-Gu, Daejeon 34929, Korea
TKM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine, Daejeon 34054, Korea
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 7 November 2015 / Revised: 18 December 2015 / Accepted: 21 December 2015 / Published: 25 December 2015
(This article belongs to the Section Natural Products Chemistry)
Full-Text   |   PDF [1984 KB, uploaded 25 December 2015]   |  


We evaluated the anti-atopic dermatitis (AD) effect of Atofreellage (AF), a herbal formula composed of 10 medicinal plants. AD was induced on the dorsal skin areas of NC/Nga mice (male, seven weeks old) by daily application of 2,4-dinitrochlorobenzene (DNCB) for five weeks. After three weeks of DNCB application, 200 μL of AF (0, 25, 50 or 100 mg/mL) was applied to the skin lesions. Histological findings, blood cell populations, serum levels of immunoglobulin E (IgE), histamine, pro-inflammatory cytokines, and inflammatory signaling in the skin tissue, and T-helper cell type 2 (Th2)-related cytokines in splenocytes were analyzed. Histopathological findings showed AF treatment notably attenuated the thickness of dorsal skin, and eosinophil infiltration. AF treatment (especially 100 mg/mL) also demonstrably ameliorated the blood cell population abnormalities, as the notable elevation of serum concentrations of IgE, histamine, TNF-α, IL-6 and IL-1β were remarkably normalized by AF treatment. Western blot analysis evidenced the apparent normalization of inflammatory signals (ERK, p38 MAP kinase, JNK, and NF-κB) in the skin tissue. Additionally, AF treatment notably attenuated the activation of Th2-dominant cytokines (IL-13, IL-4, and IL-5) in Con A-treated splenocytes in an ex vivo assay. In conclusion, this study provides experimental evidence for the clinical relevance of Atofreellage. View Full-Text
Keywords: atopic dermatitis; herbal medicine; inflammation; NC/Nga murine model atopic dermatitis; herbal medicine; inflammation; NC/Nga murine model

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Kim, W.-Y.; Kim, H.-G.; Lee, H.-W.; Lee, J.-S.; Im, H.-J.; Kim, H.-S.; Lee, S.-B.; Son, C.-G. A Herbal Formula, Atofreellage, Ameliorates Atopic Dermatitis-Like Skin Lesions in an NC/Nga Mouse Model. Molecules 2016, 21, 35.

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