Next Article in Journal
Synthesis, Characterization, Antimicrobial Screening and Free-Radical Scavenging Activity of Some Novel Substituted Pyrazoles
Next Article in Special Issue
A Selective G-Quadruplex DNA-Stabilizing Ligand Based on a Cyclic Naphthalene Diimide Derivative
Previous Article in Journal
KMUP-1 Attenuates Endothelin-1-Induced Cardiomyocyte Hypertrophy through Activation of Heme Oxygenase-1 and Suppression of the Akt/GSK-3β, Calcineurin/NFATc4 and RhoA/ROCK Pathways
Previous Article in Special Issue
Synthesis and Biological Evaluation of Novel Water-Soluble Poly-(ethylene glycol)-10-hydroxycamptothecin Conjugates
Article

Optimization of Purification, Identification and Evaluation of the in Vitro Antitumor Activity of Polyphenols from Pinus Koraiensis Pinecones

1
Harbin Institute of Technology, 73 Huanghe Road, Nangang District, Harbin 150090, China
2
Northeast Forestry University, 26 Hexing Road, Xiangfang District, Harbin 150040, China
3
Heilongjiang University of Chinese Medicine, 24 Heping Road, Xiangfang District, Harbin 150040, China
4
Jilin Medical College, 5 Jilin street, Jilin 132013, China
*
Author to whom correspondence should be addressed.
Academic Editor: Jean Jacques Vanden Eynde
Molecules 2015, 20(6), 10450-10467; https://doi.org/10.3390/molecules200610450
Received: 6 February 2015 / Revised: 12 May 2015 / Accepted: 2 June 2015 / Published: 5 June 2015
In this study, an efficient purification method for the polyphenols of Pinus koraiensis pinecone (PPP) has been developed. AB-8 resin was verified to offer good adsorption and desorption ratio for PPP. Response surface methodology (RSM) indicated that the optimized purification parameters for PPP were 1.70 mg GAE/mL phenolic sample concentration, 22.00 mL sample volume, and 63.00% ethanol concentration. Under these conditions, the experimental purity of PPP was 27.93 ± 0.14% (n = 3), which matched well with the predicted purity of 28.17%. Next, the antiproliferative effects of PPP on seven cancer cell lines, including A375 (human skin melanoma cancer cell line), A549 (human lung cancer cell line), SH-SY5Y (human neuroblastoma cell line), LOVO (human colon cancer stem cell line), MCF-7 (human breast cancer cell line), HeLa (human cervical cancer line), and HT29 (human colon cancer line), were examined by MTT assays. The results indicated that PPP had the highest capacity for inhibiting LOVO cells growth with an EC50 value of 0.317 ± 0.0476 mg/mL. Finally, Ultra-high performance liquid chromatography- tandem mass spectrometry (UPLC-MS) was used to tentatively identify twenty-four peaks in the purified PPP, of which five representative peaks were identified as catechin, methyl quercetin, o-vanillin, luteolin and coronaric acid. Our results demonstrate that Pinus koraiensis pinecone is a readily available source of polyphenols, and the purified PPP could be a promising natural antitumor agent for applications in functional foods. View Full-Text
Keywords: pinecone of Pinus koraiensis; polyphenols purification; response surface methodology; antiproliferative activity; compound identification pinecone of Pinus koraiensis; polyphenols purification; response surface methodology; antiproliferative activity; compound identification
Show Figures

Figure 1

MDPI and ACS Style

Yi, J.; Wang, Z.; Bai, H.; Yu, X.; Jing, J.; Zuo, L. Optimization of Purification, Identification and Evaluation of the in Vitro Antitumor Activity of Polyphenols from Pinus Koraiensis Pinecones. Molecules 2015, 20, 10450-10467. https://doi.org/10.3390/molecules200610450

AMA Style

Yi J, Wang Z, Bai H, Yu X, Jing J, Zuo L. Optimization of Purification, Identification and Evaluation of the in Vitro Antitumor Activity of Polyphenols from Pinus Koraiensis Pinecones. Molecules. 2015; 20(6):10450-10467. https://doi.org/10.3390/molecules200610450

Chicago/Turabian Style

Yi, Juanjuan, Zhenyu Wang, Haina Bai, Xiaojin Yu, Jing Jing, and Lili Zuo. 2015. "Optimization of Purification, Identification and Evaluation of the in Vitro Antitumor Activity of Polyphenols from Pinus Koraiensis Pinecones" Molecules 20, no. 6: 10450-10467. https://doi.org/10.3390/molecules200610450

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop