Next Article in Journal / Special Issue
BMP Signaling Regulates Bone Morphogenesis in Zebrafish through Promoting Osteoblast Function as Assessed by Their Nitric Oxide Production
Previous Article in Journal
Pi-pi Stacking Mediated Cooperative Mechanism for Human Cytochrome P450 3A4
Previous Article in Special Issue
Nitric Oxide Donors and Selective Carbonic Anhydrase Inhibitors: A Dual Pharmacological Approach for the Treatment of Glaucoma, Cancer and Osteoporosis
Article Menu

Export Article

Open AccessArticle
Molecules 2015, 20(5), 7574-7585;

Nitric Oxide Plays a Central Role in Water Stress-Induced Tanshinone Production in Salvia miltiorrhiza Hairy Roots

College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China
College of Biological Science & Engineering, Shaanxi University of Technology, Hanzhong 723000, China
Tasly R&D Institute, Tasly Holding Group Co. Ltd, Tianjin 300410, China
Tianjin Tasly Modern TCM Resources Co., Ltd., Tianjin 300402, China
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Academic Editor: Claudio Battilocchio
Received: 24 February 2015 / Revised: 7 April 2015 / Accepted: 9 April 2015 / Published: 24 April 2015
(This article belongs to the Special Issue Nitric Oxide (NO) Release Chemistry)
Full-Text   |   PDF [4846 KB, uploaded 24 April 2015]   |  


Nitric oxide (NO), a well-known signaling molecule plays an important role in abiotic and biotic stress-induced production of plant secondary metabolites. In this study, roles of NO in water stress-induced tanshinone production in Salvia miltiorrhiza hairy roots were investigated. The results showed that accumulations of four tanshinone compounds in S. miltiorrhiza hairy roots were significantly stimulated by sodium nitroprusside (SNP, a NO donor) at 100 μM. Effects of SNP were just partially arrested by the mevalonate (MVA) pathway inhibitor (mevinolin), but were completely inhibited by the 2-C-methyl-d-erythritol-4-phosphate pathway (MEP) inhibitor (fosmidomycin). The increase of tanshinone accumulation and the up-regulation of HMGR and DXR expression by PEG and ABA treatments were partially inhibited by an inhibitor of NO biosynthesis (Nω-nitro-L-arginine methyl ester (L-NAME)) and a NO scavenger (2-(4-Carboxyphenyl)- 4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO)). Simultaneously, NO generation in the hairy roots was triggered by PEG and ABA, and the effects were also arrested by c-PTIO and L-NAME. These results indicated that NO signaling probably plays a central role in water stress-induced tanshinone production in S. miltiorrhiza hairy roots. SNP mainly stimulated the MEP pathway to increase tanshinone accumulation. View Full-Text
Keywords: nitric oxide; tanshinone; Salvia miltiorrhiza; polyethylene glycol; abscisic acid nitric oxide; tanshinone; Salvia miltiorrhiza; polyethylene glycol; abscisic acid

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Du, X.; Zhang, C.; Guo, W.; Jin, W.; Liang, Z.; Yan, X.; Guo, Z.; Liu, Y.; Yang, D. Nitric Oxide Plays a Central Role in Water Stress-Induced Tanshinone Production in Salvia miltiorrhiza Hairy Roots. Molecules 2015, 20, 7574-7585.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top