Next Article in Journal
Identification of the Valid Reference Genes for Quantitative RT-PCR in Annual Ryegrass (Lolium multiflorum) under Salt Stress
Next Article in Special Issue
Flavonoids from Sideritis Species: Human Monoamine Oxidase (hMAO) Inhibitory Activities, Molecular Docking Studies and Crystal Structure of Xanthomicrol
Previous Article in Journal
QM/MM Calculations with deMon2k
Open AccessArticle

In Vitro Neuroprotective Activities of Compounds from Angelica shikokiana Makino

1
Division of Systematic Forest and Forest Products Sciences, Department of agroenvironmental sciences, Faculty of Agriculture, Graduate School of Kyushu University, Fukouka 812-8581, Japan
2
Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt
3
Department of Bioscience & Biotechnology, Graduate School of Bioresource and Bioenvironmental sciences, Kyushu University, Fukouka 812-8581, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Peter Koulen
Molecules 2015, 20(3), 4813-4832; https://doi.org/10.3390/molecules20034813
Received: 5 February 2015 / Revised: 3 March 2015 / Accepted: 9 March 2015 / Published: 16 March 2015
Angelica shikokiana is widely marketed in Japan as a dietary food supplement. With a focus on neurodegenerative conditions such as Alzheimer’s disease, the aerial part was extracted and through bio-guided fractionation, fifteen compounds [α-glutinol, β-amyrin, kaempferol, luteolin, quercetin, kaempferol-3-O-glucoside, kaempferol-3-O-rutinoside, methyl chlorogenate, chlorogenic acid, hyuganin E, 5-(hydroxymethyl)-2-furaldehyde, β-sitosterol-3-O-glucoside, adenosine (isolated for the first time from A. shikokiana), isoepoxypteryxin and isopteryxin] were isolated. Isolated compounds were evaluated for in vitro neuroprotection using acetylcholine esterase inhibitory, protection against hydrogen peroxide and amyloid β peptide (Aβ25-35)-induced neurotoxicity in neuro-2A cells, scavenging of hydroxyl radicals and intracellular reactive oxygen species and thioflavin T assays. Quercetin showed the strongest AChE inhibition (IC50 value = 35.5 µM) through binding to His-440 and Tyr-70 residues at the catalytic and anionic sites of acetylcholine esterase, respectively. Chlorogenic acid, its methyl ester, quercetin and luteolin could significantly protect neuro-2A cells against H2O2-induced neurotoxicity and scavenge hydroxyl radical and intracellular reactive oxygen species. Kaempferol-3-O-rutinoiside, hyuganin E and isoepoxypteryxin significantly decreased Aβ25-35-induced neurotoxicity and Th-T fluorescence. To the best of our knowledge, this is the first report about neuroprotection of hyuganin E and isoepoxypteryxin against Aβ25-35-induced neurotoxicity. View Full-Text
Keywords: Angelica shikokiana; neuroprotection; amyloid β peptide; hydrogen peroxide; acetylcholine esterase Angelica shikokiana; neuroprotection; amyloid β peptide; hydrogen peroxide; acetylcholine esterase
Show Figures

Graphical abstract

MDPI and ACS Style

Mira, A.; Yamashita, S.; Katakura, Y.; Shimizu, K. In Vitro Neuroprotective Activities of Compounds from Angelica shikokiana Makino. Molecules 2015, 20, 4813-4832.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop