Next Article in Journal
Identification of the Valid Reference Genes for Quantitative RT-PCR in Annual Ryegrass (Lolium multiflorum) under Salt Stress
Next Article in Special Issue
Flavonoids from Sideritis Species: Human Monoamine Oxidase (hMAO) Inhibitory Activities, Molecular Docking Studies and Crystal Structure of Xanthomicrol
Previous Article in Journal
QM/MM Calculations with deMon2k
Open AccessArticle

In Vitro Neuroprotective Activities of Compounds from Angelica shikokiana Makino

Division of Systematic Forest and Forest Products Sciences, Department of agroenvironmental sciences, Faculty of Agriculture, Graduate School of Kyushu University, Fukouka 812-8581, Japan
Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt
Department of Bioscience & Biotechnology, Graduate School of Bioresource and Bioenvironmental sciences, Kyushu University, Fukouka 812-8581, Japan
Author to whom correspondence should be addressed.
Academic Editor: Peter Koulen
Molecules 2015, 20(3), 4813-4832;
Received: 5 February 2015 / Revised: 3 March 2015 / Accepted: 9 March 2015 / Published: 16 March 2015
Angelica shikokiana is widely marketed in Japan as a dietary food supplement. With a focus on neurodegenerative conditions such as Alzheimer’s disease, the aerial part was extracted and through bio-guided fractionation, fifteen compounds [α-glutinol, β-amyrin, kaempferol, luteolin, quercetin, kaempferol-3-O-glucoside, kaempferol-3-O-rutinoside, methyl chlorogenate, chlorogenic acid, hyuganin E, 5-(hydroxymethyl)-2-furaldehyde, β-sitosterol-3-O-glucoside, adenosine (isolated for the first time from A. shikokiana), isoepoxypteryxin and isopteryxin] were isolated. Isolated compounds were evaluated for in vitro neuroprotection using acetylcholine esterase inhibitory, protection against hydrogen peroxide and amyloid β peptide (Aβ25-35)-induced neurotoxicity in neuro-2A cells, scavenging of hydroxyl radicals and intracellular reactive oxygen species and thioflavin T assays. Quercetin showed the strongest AChE inhibition (IC50 value = 35.5 µM) through binding to His-440 and Tyr-70 residues at the catalytic and anionic sites of acetylcholine esterase, respectively. Chlorogenic acid, its methyl ester, quercetin and luteolin could significantly protect neuro-2A cells against H2O2-induced neurotoxicity and scavenge hydroxyl radical and intracellular reactive oxygen species. Kaempferol-3-O-rutinoiside, hyuganin E and isoepoxypteryxin significantly decreased Aβ25-35-induced neurotoxicity and Th-T fluorescence. To the best of our knowledge, this is the first report about neuroprotection of hyuganin E and isoepoxypteryxin against Aβ25-35-induced neurotoxicity. View Full-Text
Keywords: Angelica shikokiana; neuroprotection; amyloid β peptide; hydrogen peroxide; acetylcholine esterase Angelica shikokiana; neuroprotection; amyloid β peptide; hydrogen peroxide; acetylcholine esterase
Show Figures

Graphical abstract

MDPI and ACS Style

Mira, A.; Yamashita, S.; Katakura, Y.; Shimizu, K. In Vitro Neuroprotective Activities of Compounds from Angelica shikokiana Makino. Molecules 2015, 20, 4813-4832.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

Only visits after 24 November 2015 are recorded.
Search more from Scilit
Back to TopTop