The Toxicity Mechanisms of Action of Aβ25–35 in Isolated Rat Cardiac Myocytes
Abstract
:1. Introduction
2. Results
2.1. Effect of Aβ25–35 on the Viability of Rat Cardiac Myocyte in Vitro
2.2. Aβ25–35 Induced Cardiac Myocytes Apoptosisfigure
2.3. Aβ25–35 Induced ER Stress in Cardiac Myocytes Cell
2.4. Aβ25–35 Affects the Cytoskeleton Assembly in Cardiac Myocyte Cells
2.5. Activation of p38 and ERK1/2 by Aβ25–35 in Cardiac Myocytes Cell
3. Discussion
4. Experimental
4.1. Cells and Culture Conditions
4.2. Reagents
4.3. Viability Assay
4.4. Cell Morphology Assessment
4.5. TUNEL-Based Assay
4.6. Apoptosis Assay
4.7. Immunofluorescence Assays
4.8. Immunoblotting
4.9. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Zhang, B.; Bian, X.; He, P.; Fu, X.; Higuchi, K.; Yang, X.; Li, D. The Toxicity Mechanisms of Action of Aβ25–35 in Isolated Rat Cardiac Myocytes. Molecules 2014, 19, 12242-12257. https://doi.org/10.3390/molecules190812242
Zhang B, Bian X, He P, Fu X, Higuchi K, Yang X, Li D. The Toxicity Mechanisms of Action of Aβ25–35 in Isolated Rat Cardiac Myocytes. Molecules. 2014; 19(8):12242-12257. https://doi.org/10.3390/molecules190812242
Chicago/Turabian StyleZhang, Beiru, Xiaohui Bian, Ping He, Xiaoying Fu, Keiichi Higuchi, Xu Yang, and Detian Li. 2014. "The Toxicity Mechanisms of Action of Aβ25–35 in Isolated Rat Cardiac Myocytes" Molecules 19, no. 8: 12242-12257. https://doi.org/10.3390/molecules190812242