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Molecules 2014, 19(8), 10863-10876;

Characterization and Antitumor Activity of a Polysaccharide from Sarcodia ceylonensis

Department of Landscape Plants, Sichuan Agriculture University, Chengdu 611130, China
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 30 May 2014 / Revised: 14 July 2014 / Accepted: 15 July 2014 / Published: 25 July 2014
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A water-soluble polysaccharide from Sarcodia ceylonensis was obtained by using the method of water-extraction and ethanol-precipitation. The polysaccharide was further purified by chromatography on AB-8 and ADS-7 columns, yielding a pure polysaccharide termed SCP-60. The molecular weight (Mw) of SCP-60 was calculated to be 50.0 kDa, based on the calibration curve obtained with a series of Dextran T standards. The results of FT-IR indicated that the polysaccharide contains the α-configuration of sugar units. GC-MS analysis revealed that SCP-60 was mainly composed of galactose and glucose. NMR spectroscopy revealed SCP-60 had the backbone consisting of →6)-α-Manp-(1→, α-d-Glcp-(1→, →6)-α-d-Glcp-(1→ and →6)-α-Galp-(1→. In order to evaluate the antitumor activity in vivo of the polysaccharide, a sarcoma 180 model was used. The results showed SCP-60 had strong antitumor ability, meanwhile, SCP-60 at a high dose (100 mg/kg) could significantly increase the thymic and splenic indices of S180 mice, and strongly promote the secretion of IL-2, TNF-α and IFN-γ, increase the SOD activities and reduce the concentrations of MDA in blood. Therefore the polysaccharide SCP-60 should be explored as a novel potential antitumor drug. View Full-Text
Keywords: Sarcodia ceylonensis; polysaccharide; antitumor; purification Sarcodia ceylonensis; polysaccharide; antitumor; purification

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Fan, Y.; Lin, M.; Luo, A.; Chun, Z.; Luo, A. Characterization and Antitumor Activity of a Polysaccharide from Sarcodia ceylonensis. Molecules 2014, 19, 10863-10876.

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