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Molecules 2014, 19(7), 9850-9863;

Preparation of Polyphosphazene Hydrogels for Enzyme Immobilization

MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China
Author to whom correspondence should be addressed.
Received: 19 May 2014 / Revised: 23 June 2014 / Accepted: 24 June 2014 / Published: 8 July 2014
(This article belongs to the Special Issue Enzyme Immobilization)
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We report on the synthesis and application of a new hydrogel based on a methacrylate substituted polyphosphazene. Through ring-opening polymerization and nucleophilic substitution, poly[bis(methacrylate)phosphazene] (PBMAP) was successfully synthesized from hexachlorocyclotriphosphazene. By adding PBMAP to methacrylic acid solution and then treating with UV light, we could obtain a cross-linked polyphosphazene network, which showed an ultra-high absorbency for distilled water. Lipase from Candida rugosa was used as the model lipase for entrapment immobilization in the hydrogel. The influence of methacrylic acid concentration on immobilization efficiency was studied. Results showed that enzyme loading reached a maximum of 24.02 mg/g with an activity retention of 67.25% when the methacrylic acid concentration was 20% (w/w). View Full-Text
Keywords: polyphosphazene hydrogel; swelling; lipase; entrapment; activity polyphosphazene hydrogel; swelling; lipase; entrapment; activity

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Qian, Y.-C.; Chen, P.-C.; He, G.-J.; Huang, X.-J.; Xu, Z.-K. Preparation of Polyphosphazene Hydrogels for Enzyme Immobilization. Molecules 2014, 19, 9850-9863.

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