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Molecules 2014, 19(7), 8890-8903;

Using MUSIC and CC(CO)NH for Backbone Assignment of Two Medium-Sized Proteins Not Fully Accessible to Standard 3D NMR

Department of Chemistry, University of Bergen, PO Box 7800, 5020 Bergen, Norway
Department of Clinical Science, University of Bergen, PO Box 7804, 5020 Bergen, Norway
Author to whom correspondence should be addressed.
Received: 5 May 2014 / Revised: 5 June 2014 / Accepted: 12 June 2014 / Published: 26 June 2014
(This article belongs to the Special Issue Design and Study of Kinase Inhibitors)
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The backbone assignment of medium-sized proteins is rarely as straightforward as that of small proteins, and thus often requires creative solutions. Here, we describe the application of a combination of standard 3D heteronuclear methods with CC(CO)NH and a variety of MUltiplicity Selective In-phase Coherence transfer (MUSIC) experiments. Both CC(CO)NH and MUSIC are, in theory, very powerful methods for the backbone assignment of proteins. Due to low sensitivity, their use has usually been linked to small proteins only. However, we found that combining CC(CO)NH and MUSIC experiments simplified the assignment of two challenging medium-sized proteins of 13 and 19.5 kDa, respectively. These methods are to some extent complementary to each other: CC(CO)NH acquired with a long isotropic mixing time can identify amino acids with large aliphatic side chains. Whereas the most sensitive MUSIC experiments identify amino acid types that cannot be detected by CC(CO)NH, comprising the residues with acid and amide groups, and aromatic rings in their side chains. Together these methods provide a means of identifying the majority of peaks in the 2D 15N HSQC spectrum which simplifies the backbone assignment work even for proteins, e.g., small kinases, whose standard spectra resulted in little spectral resolution and low signal intensities. View Full-Text
Keywords: protein NMR spectroscopy; backbone assignment; CC(CO)NH; MUSIC protein NMR spectroscopy; backbone assignment; CC(CO)NH; MUSIC

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Brenner, A.K.; Frøystein, N.Å. Using MUSIC and CC(CO)NH for Backbone Assignment of Two Medium-Sized Proteins Not Fully Accessible to Standard 3D NMR. Molecules 2014, 19, 8890-8903.

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