Evaluation of Styrene-Divinylbenzene Beads as a Support to Immobilize Lipases
AbstractA commercial and very hydrophobic styrene-divinylbenzene matrix, MCI GEL® CHP20P, has been compared to octyl-Sepharose® beads as support to immobilize three different enzymes: lipases from Thermomyces lanuginosus (TLL) and from Rhizomucor miehie (RML) and Lecitase® Ultra, a commercial artificial phospholipase. The immobilization mechanism on both supports was similar: interfacial activation of the enzymes versus the hydrophobic surface of the supports. Immobilization rate and loading capacity is much higher using MCI GEL® CHP20P compared to octyl-Sepharose® (87.2 mg protein/g of support using TLL, 310 mg/g using RML and 180 mg/g using Lecitase® Ultra). The thermal stability of all new preparations is much lower than that of the standard octyl-Sepharose® immobilized preparations, while the opposite occurs when the inactivations were performed in the presence of organic co-solvents. Regarding the hydrolytic activities, the results were strongly dependent on the substrate and pH of measurement. Octyl-Sepharose® immobilized enzymes were more active versus p-NPB than the enzymes immobilized on MCI GEL® CHP20P, while RML became 700-fold less active versus methyl phenylacetate. Thus, the immobilization of a lipase on this matrix needs to be empirically evaluated, since it may present very positive effects in some cases while in other cases it may have very negative ones. View Full-Text
Share & Cite This Article
Garcia-Galan, C.; Barbosa, O.; Hernandez, K.; Santos, J.C.S.; Rodrigues, R.C.; Fernandez-Lafuente, R. Evaluation of Styrene-Divinylbenzene Beads as a Support to Immobilize Lipases. Molecules 2014, 19, 7629-7645.
Garcia-Galan C, Barbosa O, Hernandez K, Santos JCS, Rodrigues RC, Fernandez-Lafuente R. Evaluation of Styrene-Divinylbenzene Beads as a Support to Immobilize Lipases. Molecules. 2014; 19(6):7629-7645.Chicago/Turabian Style
Garcia-Galan, Cristina; Barbosa, Oveimar; Hernandez, Karel; Santos, Jose C.S.; Rodrigues, Rafael C.; Fernandez-Lafuente, Roberto. 2014. "Evaluation of Styrene-Divinylbenzene Beads as a Support to Immobilize Lipases." Molecules 19, no. 6: 7629-7645.