Antiproliferative Activity and Induction of Apoptosis in PC-3 Cells by the Chalcone Cardamonin from Campomanesia adamantium (Myrtaceae) in a Bioactivity-Guided Study
Abstract
:1. Introduction
2. Results and Discussion
2.1. Extraction and Isolation
2.2. Quantification by UPLC-MS
2.3. Cardamonin, Fruit and Leaf Extracts Inhibited Survival of Cultured Human PC-3 Prostate Cancer Cells
2.4. Cardamonin Decreases NF-kB1 Expression Measured by qRT-PCR in Cultured PC-3 Cells
2.5. Flow Cytometric Analysis of DNA Fragmentation in Cultured Human PC-3 Prostate cancer Cells
3. Experimental
3.1. General
3.2. Plant Material
3.3. Extraction and Isolation
3.4. UPLC-MS Quantification
3.5. Antiproliferative Assay
3.6. Analysis of NF-kB1 Expression by qRT-PCR
3.7. DNA Fragmentation Analysis by Flow Cytometry
3.8. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Pascoal, A.C.R.F.; Ehrenfried, C.A.; Lopez, B.G.-C.; De Araujo, T.M.; Pascoal, V.D.B.; Gilioli, R.; Anhê, G.F.; Ruiz, A.L.T.G.; Carvalho, J.E.d.; Stefanello, M.É.A.; et al. Antiproliferative Activity and Induction of Apoptosis in PC-3 Cells by the Chalcone Cardamonin from Campomanesia adamantium (Myrtaceae) in a Bioactivity-Guided Study. Molecules 2014, 19, 1843-1855. https://doi.org/10.3390/molecules19021843
Pascoal ACRF, Ehrenfried CA, Lopez BG-C, De Araujo TM, Pascoal VDB, Gilioli R, Anhê GF, Ruiz ALTG, Carvalho JEd, Stefanello MÉA, et al. Antiproliferative Activity and Induction of Apoptosis in PC-3 Cells by the Chalcone Cardamonin from Campomanesia adamantium (Myrtaceae) in a Bioactivity-Guided Study. Molecules. 2014; 19(2):1843-1855. https://doi.org/10.3390/molecules19021843
Chicago/Turabian StylePascoal, Aislan Cristina Rheder Fagundes, Carlos Augusto Ehrenfried, Begoña Gimenez-Cassina Lopez, Thiago Matos De Araujo, Vinicius D’ávila Bitencourt Pascoal, Rovilson Gilioli, Gabriel Forato Anhê, Ana Lúcia Tasca Goes Ruiz, João Ernesto de Carvalho, Maria Élida Alves Stefanello, and et al. 2014. "Antiproliferative Activity and Induction of Apoptosis in PC-3 Cells by the Chalcone Cardamonin from Campomanesia adamantium (Myrtaceae) in a Bioactivity-Guided Study" Molecules 19, no. 2: 1843-1855. https://doi.org/10.3390/molecules19021843