Phenolic Antioxidants Isolated from the Flowers of Osmanthus fragrans
Abstract
:1. Introduction
2. Results and Discussion
2.1. Antioxidative Activity and Total Phenolic Content of Extracts
DPPH scavenging effects | H2O2 scavenging effects | ||
---|---|---|---|
IC50 (μg/mL) | IC50 (μg/mL) | ||
MeOH extract | 12.8 | 16.6 | |
CHCl3 sub-extract | 20.9 | nd |
2.2. Isolation and Structural Characterization of Phenolics
2.3. Evaluation of Antioxidative Activity of Isolated Phenolics
2.3.1. DPPH Radical Scavenging Activity
2.3.2. Hydrogen Peroxide Scavenging Activity
DPPH scavenging effects | H2O2 scavenging effects | |||
---|---|---|---|---|
IC50 (μM) | IC50 (μM) | |||
(+)-phillygenin (2) | 19.1 | 10.5 | ||
Rutin (4) | 10.3 | 23.4 | ||
Verbascoside (5) | 6.2 | 13.4 | ||
Quercetin | 7.3 | 15.9 | ||
Gallic acid | 27.0 | 8.2 | ||
Trolox | 19.6 | 17.2 |
3. Experimental
3.1. General
3.2. Plant Material
3.3. Extraction of Seven Flowers and Green Tea
3.4. Extraction and Isolation of Osmanthus fragrans
3.5. Evaluation of DPPH Radical Scavenging Activity
3.5.1. DPPH Radical Scavenging Assay
3.5.2. Hydrogen Peroxide Scavenging Assay
3.6. Determination of Total Phenolic Content
3.7. Statistical Analysis
4. Conclusions
Acknowledgments
References
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Hung, C.-Y.; Tsai, Y.-C.; Li, K.-Y. Phenolic Antioxidants Isolated from the Flowers of Osmanthus fragrans. Molecules 2012, 17, 10724-10737. https://doi.org/10.3390/molecules170910724
Hung C-Y, Tsai Y-C, Li K-Y. Phenolic Antioxidants Isolated from the Flowers of Osmanthus fragrans. Molecules. 2012; 17(9):10724-10737. https://doi.org/10.3390/molecules170910724
Chicago/Turabian StyleHung, Chien-Ya, Yu-Cheng Tsai, and Kuo-Yu Li. 2012. "Phenolic Antioxidants Isolated from the Flowers of Osmanthus fragrans" Molecules 17, no. 9: 10724-10737. https://doi.org/10.3390/molecules170910724
APA StyleHung, C.-Y., Tsai, Y.-C., & Li, K.-Y. (2012). Phenolic Antioxidants Isolated from the Flowers of Osmanthus fragrans. Molecules, 17(9), 10724-10737. https://doi.org/10.3390/molecules170910724