Neuroprotective Activity of Lavender Oil on Transient Focal Cerebral Ischemia in Mice
Abstract
:1. Introduction
2. Results and Discussion
2.1. Neurological Deficits and Infarct Area
Group | Dose (mg/kg) | Neurological deficit scores | Infarct area (%) |
---|---|---|---|
Vehicle | / | 2.20 ± 0.79 | 30.14 ± 3.83 |
Sham | / | 0.00 ± 0.00 ** | 0.00 ± 0.00 ** |
Lavender oil | 50 | 2.10 ± 0.74 | 28.11 ± 3.41 |
Lavender oil | 100 | 2.00 ± 0.82 | 24.85 ± 2.30 * |
Lavender oil | 200 | 1.50 ± 0.71 | 15.10 ± 2.93 ** |
Edaravone | 3 | 1.40 ± 0.52 * | 11.01 ± 1.42 ** |
2.2. Histopathology (H&E) Staining
2.3. Protein Carbonyl Content (PCC) Decreased in Lavender Oil-Treated Groups
2.4. Lavender Oil-Treatment Decreased Malondialdehyde (MDA) Content
2.5. Lavender Oil-Treatment Alleviate the Decrease of Antioxidant Enzyme Activities Induced by IR
Group | Dose (mg/kg) | CAT (U/mg protein) | SOD (U/mg protein) | GSH-Px (U/mg protein) |
---|---|---|---|---|
Vehicle | / | 28.38 ± 10.62 | 5.90 ± 1.19 | 27.04 ± 4.24 |
Sham | / | 69.58 ± 13.25 ** | 12.64 ± 0.78 ** | 45.13 ± 3.87 ** |
Lavender oil | 50 | 32.10 ± 8.36 | 6.22 ± 0.91 | 29.21 ± 4.15 |
Lavender oil | 100 | 35.40 ± 5.78 | 6.73 ± 0.81 | 33.11 ± 3.77 * |
Lavender oil | 200 | 39.92 ± 5.75 * | 7.68 ± 0.76 * | 34.02 ± 3.51 * |
Edaravone | 3 | 56.54 ± 6.50 ** | 10.76 ± 0.54 ** | 37.99 ± 3.05 ** |
2.6. Lavender Oil-Treatment Alleviate the Decrease of GSH/GSSG Ratio Induced by IR
2.7. Lavender Oil-Treatment Decreased Reactive ROS Formation in Brain Mitochondria
2.8. Discussion
3. Experimental
3.1. Animals
3.2. Chemicals and Reagents
3.3. Drug Administration and Surgical Procedure
3.4. Measurement of Neurological Deficits
3.5. Measurement of Infarct Area
3.6. Measurement of Histopathological Changes
3.7. Biochemical Measurements
3.7.1. Tissue Preparation
3.7.2. Measurement of Protein Carbonyl Content
3.7.3. Measurement of Malondialdehyde (MDA) Content
3.7.4. Measurement of Superoxide Dismutase (SOD) Activity
3.7.5. Measurement of Catalase (CAT) Activity
3.7.6. Measurement of Glutathione Peroxidase (GSH-Px) Activity
3.7.7. Measurement of Protein Content
3.7.8. Measurement of GSH/GSSG Ratio
3.7.9. Measurement of Reactive Oxygen Species (ROS) in Brain Mitochondria
3.7.10. Statistical Analysis
4. Conclusions
Acknowledgments
References
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Wang, D.; Yuan, X.; Liu, T.; Liu, L.; Hu, Y.; Wang, Z.; Zheng, Q. Neuroprotective Activity of Lavender Oil on Transient Focal Cerebral Ischemia in Mice. Molecules 2012, 17, 9803-9817. https://doi.org/10.3390/molecules17089803
Wang D, Yuan X, Liu T, Liu L, Hu Y, Wang Z, Zheng Q. Neuroprotective Activity of Lavender Oil on Transient Focal Cerebral Ischemia in Mice. Molecules. 2012; 17(8):9803-9817. https://doi.org/10.3390/molecules17089803
Chicago/Turabian StyleWang, Dong, Xuan Yuan, Ting Liu, Liangliang Liu, Yanli Hu, Zhenhua Wang, and Qiusheng Zheng. 2012. "Neuroprotective Activity of Lavender Oil on Transient Focal Cerebral Ischemia in Mice" Molecules 17, no. 8: 9803-9817. https://doi.org/10.3390/molecules17089803