Antiatherogenic Properties of Acetone Extract of Alpinia zerumbet Seeds
Abstract
:1. Introduction
2. Results and Discussion
2.1. GC-MS Analysis
Compounds | RT | Peak area (%) | |||||
---|---|---|---|---|---|---|---|
Rhizome | Stem | Leaf | Flower | Pericarp | Seed | ||
Cholestane | 13.342 | - | 0.33 | - | - | 0.20 | - |
3α,7β-Dihydroxy-5β,6β-epoxycholestane | 17.442 | - | - | - | - | 0.17 | 0.65 |
Cholest-4-ene-3,6-dione | 21.067 | - | - | - | - | - | 1.84 |
Cholest-5-en-3-ol | 21.125 | - | - | - | 0.12 | - | - |
Ergost-5-en-3-ol | 21.133 | - | - | - | - | 0.29 | - |
Sitosterol | 21.333 | 8.25 | 14.49 | 2.86 | - | 0.11 | - |
Cholestenone | 21.567 | - | - | - | - | 0.96 | - |
5α-Ergost-8(14)-ene | 21.758 | - | - | 0.49 | - | - | - |
9,19-Cyclolanostan-3-ol | 21.983 | - | - | 0.38 | - | - | - |
Cholest-4-en-3-ol | 22.075 | - | - | - | - | 0.27 | - |
Stigmasterol | 22.225 | - | 2.70 | - | 1.46 | 1.72 | - |
Cholest-8-ene-3,6-diol | 22.725 | 0.28 | 0.16 | 0.16 | - | - | - |
4,22-Stigmastadiene-3-one | 22.750 | - | - | - | - | 0.25 | - |
Campesterol | 24.067 | - | 1.55 | - | 0.35 | - | - |
2.2. Anti-Tyrosinase Activity
Sample | 50% of Inhibition (μg/mL) | |||
---|---|---|---|---|
Tyrosinase | Pancreatic lipase | 15-Lipoxygenase | LDL oxidation | |
Rhizomes | 224.53 ± 4.34 d 1 | 277.75 ± 1.60 e | 142.15 ± 1.44 c | 164.90 ± 0.71 b |
Stems | 312.53 ± 1.43 e | 747.09 ± 2.34 f | 1866.75 ± 9.12 f | 443.97 ± 3.64 d |
Leaves | 152.23 ± 1.66 b | 58.88 ± 1.55 b | 759.59 ± 3.13 d | 507.59 ± 3.73 e |
Flowers | 210.33 ± 1.76 c | 76.73 ± 0.82 c | 1356.57 ± 0.52 e | 423.43 ± 3.47 c |
Pericarps | 151.31 ± 0.26 b | 256.30 ± 3.12 d | 87.90 ± 1.60 b | 515.48 ± 2.14 e |
Seeds | 2.30 ± 0.02 a | 5.00 ± 0.70 a | 1.26 ± 0.07 a | 15.40 ± 0.86 a |
2.2. Pancreatic Lipase Inhibition
2.3. 15-Lipoxygenase Inhibition
2.4. LDL Oxidation Inhibition
2.5. Estrogenic Activity
2.6. Discussion
3. Experimental
3.1. General
3.2. Plant Material and Preparation of Extracts
3.3. GS-MS Analysis
3.4. Anti-Tyrosinase Activity Assay
3.5. Pancreatic Lipase Inhibition Assay
3.6. 15-Lipoxygenase Inhibition Assay
3.7. LDL Oxidation Inhibition Assay
3.8. Estrogenic Activity Assay
3.9. Statistical Analysis
4. Conclusion
References and Notes
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Chompoo, J.; Upadhyay, A.; Gima, S.; Fukuta, M.; Tawata, S. Antiatherogenic Properties of Acetone Extract of Alpinia zerumbet Seeds. Molecules 2012, 17, 6237-6248. https://doi.org/10.3390/molecules17066237
Chompoo J, Upadhyay A, Gima S, Fukuta M, Tawata S. Antiatherogenic Properties of Acetone Extract of Alpinia zerumbet Seeds. Molecules. 2012; 17(6):6237-6248. https://doi.org/10.3390/molecules17066237
Chicago/Turabian StyleChompoo, Jamnian, Atul Upadhyay, Shinichi Gima, Masakazu Fukuta, and Shinkichi Tawata. 2012. "Antiatherogenic Properties of Acetone Extract of Alpinia zerumbet Seeds" Molecules 17, no. 6: 6237-6248. https://doi.org/10.3390/molecules17066237