Effect of Paeonol on Antioxidant and Immune Regulatory Activity in Hepatocellular Carcinoma Rats
Abstract
:1. Introduction
2. Results and Discussion
Group | Number (n) | Death (n) | Survival rate (%) |
---|---|---|---|
I | 10 | 0 | 100 |
II | 10 | 2 | 80 |
III | 10 | 1 | 90 |
IV | 10 | 0 | 100 |
V | 10 | 0 | 100 |
VI | 10 | 0 | 100 |
Group | AST (U/L) | ALP (U/L) | ALT (U/L) | GGT (U/L) | AFU (U/L) |
---|---|---|---|---|---|
I | 288.82 ± 18.19 | 141.61 ± 9.62 | 63.55 ± 3.04 | 17.57 ± 1.75 | 30.14 ± 2.77 |
II | 392.16 ± 20.66 b | 201.17 ± 12.43 b | 91.14 ± 4.01 b | 121.63 ± 8.63 b | 174.11 ± 9.05 b |
III | 351.32 ± 19.18 d | 180.12 ± 8.92 d | 79.14 ± 3.01 d | 97.45 ± 5.11 d | 152.09 ± 9.29 d |
IV | 331.24 ± 24.16 d | 156.22 ± 6.84 d | 70.31 ± 2.81 d | 66.43 ± 3.96 d | 111.27 ± 7.08 d |
V | 297.63 ± 15.11 d | 126.18 ± 7.84 d | 64.92 ± 2.99 d | 48.12 ± 2.74 d | 81.36 ± 6.83 d |
VI | 289.68 ± 19.51 d | 132.35 ± 9.59 d | 60.26 ± 3.85 d | 33.71 ± 2.94 d | 65.62 ± 5.47 d |
Group | WBC (×109/L) | TP (g/L) | ALB (g/L) | A/G |
---|---|---|---|---|
I | 30.11 ± 1.83 | 66.39 ± 1.92 | 38.29 ± 1.72 | 1.71 ± 0.09 |
II | 22.17 ± 1.08 b | 59.18 ± 1.68 b | 34.15 ± 1.81 b | 1.53 ± 0.11 b |
III | 25.81 ± 1.22 c | 62.71 ± 1.99 c | 35.05 ± 1.83 | 1.55 ± 0.09 |
IV | 27.84 ± 1.65 c | 63.92 ± 2.02 c | 35.81 ± 1.29 | 1.64 ± 0.06 d |
V | 29.62 ± 1.09 d | 67.16 ± 2.15 d | 37.21 ± 1.47 c | 1.69 ± 0.96 d |
VI | 27.37 ± 1.11 d | 70.13 ± 2.74 d | 39.84 ± 1.61 c | 1.85 ± 0.88 d |
Group | TNF-α (pg/mL) | IFN-γ (pg/mL) |
---|---|---|
I | 613.72 ± 28.72 | 619.48 ± 24.91 |
II | 972.38 ± 52.38 b | 328.32 ± 15.51 b |
III | 904.22 ± 42.57 a | 420.24 ± 18.94 d |
IV | 811.37 ± 38.42 d | 512.33 ± 19.66 d |
V | 677.83 ± 32.64 d | 592.83 ± 25.62 d |
VI | 614.52 ± 42.52 d | 631.7 ± 39.32 d |
Group | CD4+ (%) | CD8+ (%) | CD4+/CD8+ |
---|---|---|---|
I | 48.08 ± 4.19 | 25.86 ± 1.76 | 1.85 ± 0.24 |
II | 31.47 ± 2.76 b | 33.16 ± 1.95 b | 0.94 ± 0.39 a |
III | 39.52 ± 2.72 c | 24.76 ± 1.38 d | 1.59 ± 0.22 c |
IV | 40.13 ± 3.82 c | 25.15 ± 1.81 d | 1.59 ± 0.28 c |
V | 39.21 ± 4.52 c | 24.17 ± 2.83 d | 1.62 ± 0.23 c |
VI | 37.58 ± 4.81 c | 22.13 ± 2.21 d | 1.68 ± 0.34 c |
Group | MDA(nmol/mg prot) | GSH (nmol/mg) | SOD (U/mg prot) | CAT (U/mg prot) | GSH-Px (U/mg prot) | GR (U/mg prot) |
---|---|---|---|---|---|---|
I | 3.53 ± 0.16 | 74.39 ± 3.61 | 321.32 ± 15.05 | 63.28 ± 2.11 | 51.81 ± 1.77 | 37.24 ± 1.42 |
II | 7.84 ± 0.39 b | 39.19 ± 1.74 b | 161.19 ± 11.39 b | 32.14 ± 1.72 b | 26.02 ± 1.41 b | 16.93 ± 1.39 b |
III | 5.98 ± 0.22 d | 52.77 ± 1.91 d | 199.48 ± 11.52 d | 44.17 ± 1.93 d | 35.63 ± 1.62 d | 27.18 ± 1.66 d |
IV | 4.77 ± 0.17 d | 67.14 ± 2.82 d | 267.14 ± 11.62 d | 58.11 ± 2.72 d | 45.28 ± 1.83 d | 34.14 ± 1.95 d |
V | 3.68 ± 0.21 d | 75.03 ± 2.92 d | 316.13 ± 14.71 d | 62.63 ± 2.99 d | 52.33 ± 2.41 d | 38.05 ± 1.51 d |
VI | 5.05 ± 0.29 d | 69.41 ± 2.77 d | 287.48 ± 19.66 d | 58.47 ± 2.81 d | 48.51 ± 2.73 d | 35.62 ± 1.88 d |
3. Experimental
3.1. Material
3.2. Experimental Design
3.3. Bichemical Analysis
3.4. Catalase (CAT) Measurement
3.5. Histological Analysis
3.6. Statistical Analysis
4. Conclusions
- Sample Availability: Samples of the compounds are available from the authors.
References and Notes
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Chen, B.; Ning, M.; Yang, G. Effect of Paeonol on Antioxidant and Immune Regulatory Activity in Hepatocellular Carcinoma Rats. Molecules 2012, 17, 4672-4683. https://doi.org/10.3390/molecules17044672
Chen B, Ning M, Yang G. Effect of Paeonol on Antioxidant and Immune Regulatory Activity in Hepatocellular Carcinoma Rats. Molecules. 2012; 17(4):4672-4683. https://doi.org/10.3390/molecules17044672
Chicago/Turabian StyleChen, Bendong, Mingliang Ning, and Guangshun Yang. 2012. "Effect of Paeonol on Antioxidant and Immune Regulatory Activity in Hepatocellular Carcinoma Rats" Molecules 17, no. 4: 4672-4683. https://doi.org/10.3390/molecules17044672
APA StyleChen, B., Ning, M., & Yang, G. (2012). Effect of Paeonol on Antioxidant and Immune Regulatory Activity in Hepatocellular Carcinoma Rats. Molecules, 17(4), 4672-4683. https://doi.org/10.3390/molecules17044672