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Cleavage of Oligonucleotides Containing a P3’→N5’ Phosphoramidate Linkage Mediated by Single-Stranded Oligonucleotide Templates
Open AccessArticle

Template Directed Reversible Photochemical Ligation of Oligodeoxynucleotides

1
School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa 923-1292, Japan
2
Department of Synthetic Chemistry and Biological Chemistry, Factly of Enginnering, Kyoto University, Kyoto 606-8501, Japan
3
Research Center for Bio-Architecture, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa 923-1292, Japan
*
Author to whom correspondence should be addressed.
Molecules 2012, 17(1), 163-178; https://doi.org/10.3390/molecules17010163
Received: 29 November 2011 / Revised: 21 December 2011 / Accepted: 22 December 2011 / Published: 27 December 2011
(This article belongs to the Special Issue DNA-Templated Synthesis)
We demonstrated that 5-vinyldeoxyuridine (VU) and 5-carboxyvinyldeoxyuridine (CVU) can be used to photoligate a longer oligonucleotide (ODN) from smaller ODNs on a template. By performing irradiation at 366 nm, these artificial nucleotides make photoligated ODNs with high efficiency without any side reactions. Moreover, by performing irradiation at 312 nm, these photoligated ODNs were reversed to the original ODN. VU needs to be irradiated 366 nm for 6 h, but CVU needs to be irradiated at 366 nm for 15 min. Finally, we made a self-assembled structure with an ODN containing CVU and observed the photoligated ODN by photoirradiation. View Full-Text
Keywords: photochemical ligation; 5-carboxyvinyldeoxyuridine; 5-vinyldeoxyuridine; DNA nanotechnology photochemical ligation; 5-carboxyvinyldeoxyuridine; 5-vinyldeoxyuridine; DNA nanotechnology
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Nakamura, S.; Ogasawara, S.; Matuda, S.; Saito, I.; Fujimoto, K. Template Directed Reversible Photochemical Ligation of Oligodeoxynucleotides. Molecules 2012, 17, 163-178.

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