BDNF Promotes EGF-Induced Proliferation and Migration of Human Fetal Neural Stem/Progenitor Cells via the PI3K/Akt Pathway
Abstract
:1. Introduction
2. Results
2.1. Neurosphere Formation of Human NSPCs
2.2. BDNF Treatment Increased EGF-Induced Proliferation of Human NSPCs
2.3. BDNF Treatment Increased EGF-Induced Human NSPC Migration
2.4. BDNF Promotes EGF-Induced NSPC Proliferation and Migration via the PI3K/Akt Kinase Pathway
3. Discussion
4. Experimental
4.1. Primary Cell Culture Preparation
4.2. Proliferation Assay
4.3. Flow Cytometry Assay
4.4. Transwell Cell Migration Assay
4.5. Western Blotting
4.6. Statistical Analyses
5. Conclusions
Acknowledgements
References and Notes
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- Sample Availability: Not available.
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Zhang, Q.; Liu, G.; Wu, Y.; Sha, H.; Zhang, P.; Jia, J. BDNF Promotes EGF-Induced Proliferation and Migration of Human Fetal Neural Stem/Progenitor Cells via the PI3K/Akt Pathway. Molecules 2011, 16, 10146-10156. https://doi.org/10.3390/molecules161210146
Zhang Q, Liu G, Wu Y, Sha H, Zhang P, Jia J. BDNF Promotes EGF-Induced Proliferation and Migration of Human Fetal Neural Stem/Progenitor Cells via the PI3K/Akt Pathway. Molecules. 2011; 16(12):10146-10156. https://doi.org/10.3390/molecules161210146
Chicago/Turabian StyleZhang, Qi, Gang Liu, Yi Wu, Hongying Sha, Pengyue Zhang, and Jie Jia. 2011. "BDNF Promotes EGF-Induced Proliferation and Migration of Human Fetal Neural Stem/Progenitor Cells via the PI3K/Akt Pathway" Molecules 16, no. 12: 10146-10156. https://doi.org/10.3390/molecules161210146
APA StyleZhang, Q., Liu, G., Wu, Y., Sha, H., Zhang, P., & Jia, J. (2011). BDNF Promotes EGF-Induced Proliferation and Migration of Human Fetal Neural Stem/Progenitor Cells via the PI3K/Akt Pathway. Molecules, 16(12), 10146-10156. https://doi.org/10.3390/molecules161210146