Next Article in Journal
Effect of EtOH/MgCl2 Molar Ratios on the Catalytic Properties of MgCl2-SiO2/TiCl4 Ziegler-Natta Catalyst for Ethylene Polymerization
Previous Article in Journal
Inhibitory Effects of Constituents from Euphorbia lunulata on Differentiation of 3T3-L1 Cells and Nitric Oxide Production in RAW264.7 Cells
Open AccessArticle

Role of Kupffer Cells in Thioacetamide-Induced Cell Cycle Dysfunction

Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla, 42080 Pachuca de Soto, Hgo, Mexico
Instituto de Bioquímica (CSIC-UCM), Facultad de Farmacia, Ciudad Universitaria, Plaza de Ramón y Cajal S/N, 28040 Madrid, Spain
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, D.F., 07700, Mexico
Laboratorio de Bioquímica y Medicina Experimental, División de Investigación Biomédica, Centro Médico Nacional “20 de Noviembre”, ISSSTE, México, D.F., 03229, Mexico
Facultad de Odontologia, Circuito Escolar S/N, Ciudad Universitaria (UNAM), México, D.F., 04510, Mexico
Authors to whom correspondence should be addressed.
Molecules 2011, 16(10), 8319-8331;
Received: 4 August 2011 / Revised: 11 September 2011 / Accepted: 19 September 2011 / Published: 29 September 2011
(This article belongs to the Section Molecular Diversity)
It is well known that gadolinium chloride (GD) attenuates drug-induced hepatotoxicity by selectively inactivating Kupffer cells. In the present study the effect of GD in reference to cell cycle and postnecrotic liver regeneration induced by thioacetamide (TA) in rats was studied. Two months male rats, intraveously pretreated with a single dose of GD (0.1 mmol/Kg), were intraperitoneally injected with TA (6.6 mmol/Kg). Samples of blood and liver were obtained from rats at 0, 12, 24, 48, 72 and 96 h following TA intoxication. Parameters related to liver damage were determined in blood. In order to evaluate the mechanisms involved in the post-necrotic regenerative state, the levels of cyclin D and cyclin E as well as protein p27 and Proliferating Cell Nuclear Antigen (PCNA) were determined in liver extracts because of their roles in the control of cell cycle check-points. The results showed that GD significantly reduced the extent of necrosis. Noticeable changes were detected in the levels of cyclin D1, cyclin E, p27 and PCNA when compared to those induced by thioacetamide. Thus GD pre-treatment reduced TA-induced liver injury and accelerated the postnecrotic liver regeneration. These results demonstrate that Kupffer cells are involved in TA-induced liver and also in the postnecrotic proliferative liver states. View Full-Text
Keywords: gadolinium chloride; kupffer cells; thioacetamide hepatotoxicity; cell cycle; cyclins gadolinium chloride; kupffer cells; thioacetamide hepatotoxicity; cell cycle; cyclins
Show Figures

Figure 1

MDPI and ACS Style

Bautista, M.; Andres, D.; Cascales, M.; Morales-González, J.A.; Sánchez-Reus, M.I.; Madrigal-Santillán, E.; Valadez-Vega, C.; Fregoso-Aguilar, T.; Mendoza-Pérez, J.A.; Gutiérrez-Salinas, J.; Esquivel-Soto, J. Role of Kupffer Cells in Thioacetamide-Induced Cell Cycle Dysfunction. Molecules 2011, 16, 8319-8331.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

Only visits after 24 November 2015 are recorded.
Back to TopTop