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Emodin-8-O-β-D-Glucoside from Polygonum Amplexicaule D. Don var. Sinense Forb. Promotes Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells

1
College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
2
College of Pharmacy, South-Central University for Nationalities, Wuhan 430075, China
3
Renmin Hospital, Wuhan University, Wuhan 430060, China
*
Authors to whom correspondence should be addressed.
Molecules 2011, 16(1), 728-737; https://doi.org/10.3390/molecules16010728
Received: 26 November 2010 / Revised: 7 January 2011 / Accepted: 17 January 2011 / Published: 18 January 2011
Polygonum amplexicaule D. Don var. sinense Forb. (Polygonaceae) (PAF) is a famous traditional herb used to treat fractures, rheumatoid arthritis, muscle injury and pain. The present study was designed to investigate a PAF derived-chemical compound emodin-8-O-β-D-glucoside (EG) on the proliferation and differentiation of osteoblastic MC3T3-E1 cell in vitro. A compound was isolated from PAF extract by HPLC and identified as emodin-8-O-β-D-glucoside (EG) by spectroscopic methods. EG significantly promoted cell proliferation at 0.1–100 ng/mL, and increased the cell proportion in S-phase from 16.34% to 32.16%. Moreover, EG increased alkaline phosphatase (ALP) expression in MC3T3-E1 cells at the concentration from 0.1 to 100 ng/mL and inhibited PGE2 production induced by TNF-α in osteoblasts at the concentrations ranging from 10–100 ng/mL, suggesting that cell differentiation was induced in MC3T3-E1 osteoblasts. Taken together, these results indicated compound EG directly stimulated cell proliferation and differentiation of osteoblasts, therefore this study preliminarily explored the pharmacological mechanism of PAF to promote the healing of bone rheumatism and various fractures. View Full-Text
Keywords: emodin-8-O-β-D-glucoside; Polygonum amplexicaule D. Don var. sinense Forb. (Polygonaceae); proliferation; differentiation; osteoblastic MC3T3-E1 cell emodin-8-O-β-D-glucoside; Polygonum amplexicaule D. Don var. sinense Forb. (Polygonaceae); proliferation; differentiation; osteoblastic MC3T3-E1 cell
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Xiang, M.-X.; Xu, Z.; Su, H.-W.; Hu, J.; Yan, Y.-J. Emodin-8-O-β-D-Glucoside from Polygonum Amplexicaule D. Don var. Sinense Forb. Promotes Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells. Molecules 2011, 16, 728-737.

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