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Article

Feeding Deterrents against Two Grain Storage Insects from Euphorbia fischeriana

by
Zhu Feng Geng
1,
Zhi Long Liu
2,*,
Cheng Fang Wang
3,
Qi Zhi Liu
2,
Sheng Min Shen
3,
Zi Mu Liu
3,
Shu Shan Du
3,* and
Zhi Wei Deng
1
1
Analytical and Testing Center, Beijing Normal University, Beijing 100875, China
2
Department of Entomology, China Agricultural University, Haidian District, Beijing 100094, China
3
Protection and Utilization of Traditional Chinese Medicine of Beijing Area Major Laboratory, Beijing Normal University, Haidian District, Beijing 100875, China
*
Authors to whom correspondence should be addressed.
Molecules 2011, 16(1), 466-476; https://doi.org/10.3390/molecules16010466
Submission received: 17 November 2010 / Revised: 5 January 2011 / Accepted: 7 January 2011 / Published: 10 January 2011
Browse Figure
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Abstract

:
The screening of several Chinese medicinal herbs for insecticidal principles showed that Euphorbia fischeriana roots possessed significant feeding deterrent activity against two stored-product insects (Tribolium castaneum and Sitophilus zeamais). From ethanol extract, four feeding deterrents were isolated by bioassay-guided fractionation. The compounds were identified as jolkinolide B, 12-deoxyphorbol 13-(9Z)-octadecenoate 20-acetate, 17-hydroxyjolkinolide A and B on the basis of their phytochemical and spectral data. Jolkinolide B and 17-hydroxyjolkinolide B possessed strong feeding deterrent activities against S. zeamais (EC50 = 342.1 and 543.9 ppm, respectively) and T. castaneum adults (EC50 = 361.4 and 551.5 ppm, respectively). 17-Hydroxyjolkinolide A and 12-deoxyphorbol 13-(9Z)-octadecenoate 20-acetate A also exhibited feeding deterrent activity against the two grain storage insects with EC50 values of 631.9 and 884.3 ppm for S. zeamais and 656.5 and 1058.4 ppm for T. castaneum adults.

1. Introduction

Botanical pesticides have the advantage of providing novel modes of action against insects that can reduce the risk of cross-resistance as well as offering new leads for design of target-specific molecules [1,2]. During a screening program for new agrochemicals from Chinese medicinal herbs and wild plants, Euphorbia fischeriana Steud. roots (Family: Euphorbiaceae) were found to possess significant feeding deterrence activity against two stored-product insects, red flour beetles (Tribolium castaneum Herbst) and maize weevil (Sitophilus zeamais Motsch.). E. fischeriana is a perennial herbaceous plant with milky juice, distributed mainly in north China [3]. S. zeamais and T. castaneum are the most widespread and destructive primary insect pests of stored cereals [4]. Infestations not only cause significant losses due to the consumption of grains; they also result in elevated temperature and moisture conditions that lead to an accelerated growth of molds, including toxigenic species [5]. Control of stored product insects relies heavily on the use of synthetic insecticides and fumigants, which has led to problems such as disturbances of the environment, increasing costs of application, pest resurgence, pest resistance to pesticides and lethal effects on non-target organisms in addition to direct toxicity to users [6]. These problems have highlighted the need for the development of new types of selective stored product pest-control alternatives.
The dried plant roots of Euphorbia fischeriana, known as “lang-du” in traditional Chinese medicine, have long been used for the treatment of a wide range of ailments, including edema, ascites, ingestion, as well as liver and lung cancer [7,8,9]. The ethanol extracts of E. fischeriana roots were found to possess toxicity against Oncomelania snails and miracidiums of Schistosoma [10]. Aqueous extracts of E. fischeriana roots have been used to control aphids (Aphis gossypii), cabbage beetle (Colaphellus bowringi) and Pieris rapae on vegetables and rice leaf roller Cnaphalocrocis medinalis as well as spider mites [11]. Petroleum ether extracts of E. fischeriana roots possessed strong contact toxicity against carmine spider mite, Tetranychus cinnabarinus adults [12] and eggs [13]. Moreover, one formulation based on ethanol extracts of E. fischeriana roots had been evaluated in control of cabbage moth, Barathra brassicae and green peach aphids, Myzus persicae [14].
Due to the fact that it is a common Chinese herb used in medicane, the chemical constituents and bioactivities of E. fischeriana roots have been extensively studied and the known chemical constituents of this medicinal herb include monoterpenoids, flavonoids, anthraquinones, tannins, cerebrosides, glycerols, phenolics, diterpenoids, triterpenoids and steroids [15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30]. A number of abietane diterpenoids, including jolkinolide A and B, and 17-hydroxyjolkinolide A and B were isolated. Some of these diterpenoids exhibited significant antitumor activity against several tumor lines, such as prostate LNCaP, sarcoma 180, Ehrlich ascites carcinoma, and hepatocellar carcinoma in mice [31]. However, the bioactive compounds against insects have not been isolated and identified from this Chinese medicinal herb. In this paper, we report the isolation and identification of four feeding deterrents contained in E. fischeriana roots against two stored-product insects, T. castaneum and S. zeamais by bioassay-guided fractionation.

2. Results and Discussion

2.1. Isolated bioactive compounds

Four bioactive compounds were isolated and based on bioassay-guided fractionation and identified based on their spectroscopic data and comparison with literature vales. Their chemical structures are given in Figure 1.
Molecules 16 00466 g001 550
Figure 1. Structures of feeding deterrents isolated from Euphorbia fischeriana roots.
Figure 1. Structures of feeding deterrents isolated from Euphorbia fischeriana roots.
Molecules 16 00466 g001

2.2. Feeding deterrent activity

The feeding deterrent activity of the four isolated compounds against aize weevils and red flour beetles is shown in Table 1 and Table 2. Two of the pure compounds, namely 17-hydroxyjolkinolide B and jolkinolide B exhibited significant feeding deterrent activities against T. castaneum adults at a concentration of 30 ppm and above (Table 2). However, 17-hydroxyjolkinolide A and 12-deoxyphorbol 13-(9Z)-octadecenoate 20-acetate A possessed significant feeding deterrent activities against T. castaneum adults only at a concentration of 100 ppm and above (Table 2). Among the four isolated compounds, jolkinolide B showed strongest feeding deterent activities against S. zeamais and T. castaneum adults with EC50 values of 342.1 and 361.4 ppm, respectively. At the highest concentration (1,000 ppm), S. zeamais and T. castaneum adults consumed only 39.78% and 37.58% of diet contained jolkinolide B, respectively, compared with the control. However, 12-deoxyphorbol 13-(9Z)-octadecenoate 20-acetate possessed the least feeding deterrent activities against the two grain storage insects with EC50 values of 884.3 and 1058.4 ppm, respectively. For example, S. zeamais and T. castaneum adults consumed only 48.76% and 51.67% of diet contained jolkinolide B, respectively, compared with the control at the highest concentration (1,000 ppm).
Dietary 17 hydroxyjolkinolide A and B also exhibited feeding deterrent activity S. zeamais (EC50 = 631.9 and 543.9 ppm, respectively) and T. castaneum adults (EC50 = 656.6 and 551.5 ppm, respectively). When compared with the commercial feeding deterrents, azadirachtin and toosendanin, the four isolated compounds were less active against S. zeamais adults (EC50 values of azadirachtin and toosendanin were 57 and 100 ppm, respectively) and T. castaneum adults (EC50 values of azadirachtin and toosendanin were 3 and 66 ppm, respectively) [38,39].
Table
Table 1. Feeding deterrent activities of the pure compounds isolated from E. fischeriana against S. zeamais adults.
Table 1. Feeding deterrent activities of the pure compounds isolated from E. fischeriana against S. zeamais adults.
CompoundsConcentration (ppm)Consumption of diet* (% control ± SD)EC50 (95% FL)Slope±SDChi square (χ2)
Control 100.0 ± 4.87a--
17-Hydroxyjolkinolide A100047.02 ± 2.05e
30061.53 ± 2.91d631.92.77 ± 0.1548.42
10070.96 ± 1.85c(567.2-697.4)
3090.82 ± 3.34b
1099.45 ± 2.17a
17-Hydroxyjolkinolide B100043.79 ± 3.89e
30056.07 ± 3.73d543.92.30 ± 0.1017.49
10072.89 ± 2.62c(494.7-603.8)
3082.87 ± 2.83b
1094.18 ± 3.45a
Jolkinolide B100039.78 ± 2.62e
30048.57 ± 2.18d342.12.23 ± 0.0926.91
10063.84 ± 2.96c(296.3-393.5)
3081.01 ± 4.21b
1094.23 ± 3.48a
12-Deoxyphorbol 13-(9Z)-octadecenoate 20-acetate100048.76 ± 1.89e
30068.43 ± 2.85d884.32.87 ± 0.1215.08
10080.27 ± 2.56c(787.3-965.4)
3091.09 ± 2.66b
1098.97 ± 1.65a
* Multiple range test using Tukey’s test (P < 0.05). Within each compound, the same letters denote treatments not significantly different from each other.
Table
Table 2. Feeding deterrent activities of the pure compounds isolated from E. fischeriana against T. castaneum adults.
Table 2. Feeding deterrent activities of the pure compounds isolated from E. fischeriana against T. castaneum adults.
CompoundsConcentration (ppm)Consumption of diet* (% control ± SD)EC50 (95% FL)Slope±SDChi square (χ2)
Control 100.00 ± 5.32a--
17-Hydroxyjolkinolide A100048.78 ± 4.45d
30059.12 ± 4.09c656.52.63 ± 0.1530.42
10076.56 ± 5.23b(593.4-703.5)
3090.81 ± 4.34a
1098.45 ± 1.96a
17-Hydroxyjolkinolide B100044.34 ± 5.56e
30056.64 ± 3.73d551.52.42 ± 0.1317.60
10072.81 ± 4.21c(489.3-606.5)
3087.43 ± 5.38b
1094.18 ± 3.45a
Jolkinolide B100037.58 ± 3.67e
30049.78 ± 4.32d361.42.24 ± 0.1229.70
10066.43 ± 5.16c(289.8-422.6)
3080.31 ± 3.45b
1096.23 ± 3.48a
12-Deoxyphorbol 13-(9Z)-octadecenoate 20-acetate100051.67 ± 4.93e
30070.67 ± 3.23c1058.41.96 ± 0.0935.94
10083.33 ± 3.08d(985.3-1145.7)
3092.28 ± 3.34a
1097.97 ± 2.65a
* Multiple range test using Tukey’s test (P < 0.05). Within each compound, the same letters denote treatments not significantly different from each other.
The concentration used in this study to observe feeding deterrent effects was much higher than for the commercially available products such as margosan-O, active at a 3.75 ppm azadirachtin level [36]. However, it was comparable with another commercially product toosendanin at 20 ppm [37]. The four compounds were evaluated for feeding deterrent activity against stored product insects for the first time.

3. Experimental

3.1. Plant material

Dried roots (10 kg) of E. fischeriana were purchased from Anguo Chinese Medicinal Herbs Market (Anguo 071200, Hebei Province, China). The roots were ground to a powder using a grinding mill (Retsch Muhle, Germany). The species was identified, and the voucher specimens (BNU-CMH-Dushuahan-2009-08-25-004) were deposited at the Herbarium (BNU) of College of Life Sciences, Beijing Normal University.

3.2. Insects

The maize weevil, S. zeamais and red flour beetle, T. castaneum were obtained from laboratory cultures maintained for the last 10 years in the dark in incubators at 28-30 °C and 70-80% relative humidity. T. castaneum was reared on wheat flour mixed with yeast (10:1, w:w) while S. zeamais was reared on whole wheat at 12-13% moisture content. Adults of the two species used in all the experiments were about 2 weeks old.

3.3. Feeding deterrent activity

A flour disk bioassay was used to direct the isolation of active compounds from E. fischeriana roots according to the method of Xie et al. [36] with some modifications [4,38]. Wheat flour (1.0 g) was ultrasonically stirred in distilled water (5 mL) and ethanol (50 µL) containing a fraction or pure compound was added. Pure compounds were first dissolved in ethanol (500 μL) and two drops of Tween-20 (approximately 50 μg) were added to the wheat flour suspension. Aliquots (200 μL) of this stirred suspension were placed on the bottom of a polystyrene Petri dish to form disks. The pipette was fitted with a disposable tip that had an opening enlarged to about 2 mm internal diameter by cutting about 1 cm from the bottom of the tip with a razor blade. The same amounts of ethanol and Tween-20 were applied to produce the control flour disks. The flour disks were left in the fume-hood overnight to air dry. The flour disks were then transferred to an incubator to equilibrate at 28-30 °C and 70-80% R.H. for 48 h. Each flour disk weighed between 36 and 39 mg. The moisture content of the disk was determined to be 13.5 ± 0.1% using the Kett’s Grain moisture tester (Model PB-1D2, Japan). The disks were placed in glass vials (diameter 2.5 cm, height 5.5 cm) for weighing. Twenty group-weighed, unsexed insects were then added to each vial prior to further weighing. All the insects were starved for 24 h before use. Six replicates were carried out for all treatments and controls. The experimental set-up was left in the incubator for 3 days. Finally, the uneaten parts of the flour disks were weighed. The insect consumption for the different test substances was compared to the control group. Glass vials containing treated flour disks but without insects were prepared to determine any decrease in weights that might have occurred due to evaporation of solvents.

3.4. Extraction and isolation of active ingredients

The powdered roots of E. fischeriana were extracted with 95% ethanol (50 L) at room temperature over a period of three weeks, and the extract was evaporated under reduced pressure using a vacuum rotary evaporator to afford a syrupy gum (256 g). This syrup was partitioned between methanol-water and petroleum ether (3 × 5,000 mL). The petroleum ether extracts were evaporated off to given a residue (38 g). The aqueous layer was re-partitioned with chloroform (3 × 5,000 mL) to provide a residue (173 g) after evaporation of chloroform. Further partitioning with ethyl acetate (3 × 5,000 mL) gave a residue (76 g) after evaporation of the solvent. The CHCl3 residue (25 g) was applied to a silica gel column (160-200 mesh, Qingdao Marine Chemical Plant, Shandong Province, China), eluting with petroleum ether containing increasing accounts of ethyl acetate (from 100:1 to 1:2) to give fourteen combined fractions according to TLC detection. Based on bioassay, Fraction 6, 8, 10 and 11 were chosen for further fractionation. Jolkonolide B (312 mg) was isolated from Fraction 6 (330 mg) after repeated purification on silica, Sephadex LH-20 (Pharmacia, Sweden) and PTLC (pre-coated GF254 plates, Qingdao Marine Chemical Plant). Fraction 8 (141 mg) was further chromatographed on silica gel column, Sephadex LH-20 as well as repeated PTLC to provide the bioactive compound which was recrystallized and determined to be 12-deoxyphorbol-13-(9Z)-octadecanoate-20-acetate (6.2 mg). 17-Hydroxyjolkinolide A (126.8 mg) was obtained from further chromatographed on silica gel TLC and Sephadex LH-20 and recrystallized from fraction 10 (409 mg). Fraction 11 (555 mg) was further chromatographed on silica gel column, Sephadex LH-20 and silica gel TLC to obtain the bioactive compound which was recrystallized and determined to be 17-hydroxyjolkinolide B (330 mg). The structures of the compounds were elucidated based on high-resolution electron impact mass spectrometry and nuclear magnetic resonance.

3.5. Apparatus

Melting points were measured on a Buchi 535. 1H- and 13C-NMR spectra were recorded on a Bruker Avance DRX 500 instrument using CDCl3 as solvent with TMS as internal standard. EIMS were determined on an ThermoQuest Trace 2000 mass spectrometer at 70 eV (probe).

3.6. Compound characterization

17-Hydroxyjolkinolide A. White needle-like crystals (CHCl3), m.p. 177-182 °C (m.p. 180-182 °C [24]), EI-MS m/z (%): 329 [M+] (5), 229 (10), 215 (11), 191 (12), 187 (22), 188 (20), 175 (58), 173 (100), 161 (19), 162 (24), 118 (17), 108 (20), 95 (23), 94 (38), 78 (45), 68 (53), 54 (42), 40 (55), C20H26O4. 1H-NMR (500 MHz) 5.60 (d, J = 4.6 Hz, 1H, H-11), 4.05 (s, 1H, H-14), 2.68 (d, J = 4.6 Hz, 1H, H-9), 4.65 (s, 2H, H-17), 0.97 (s, 3H, H-18), 0.88 (s, 3H, H-19), 0.76 (s, 3H, H-20). 13C-NMR (125 MHz) 169.2 (C-16), 147.3 (C-12), 146.6 (C-13), 127.3 (C-15), 106.5 (C-11), 61.3 (C-8), 56.5 (C-17), 54.4 (C-14), 53.5 (C-5), 51.8 (C-9), 41.5 (C-3), 41.3 (C-10), 39.9 (C-1), 34.0 (C-7), 33.6 (C-4), 33.4 (C-18), 21.9 (C-19), 20.8 (C-6), 18.4 (C-2), 14.1 (C-20). The 1H- and 13C-NMR data were in agreement with the reported data [28,31].
17-Hydroxyjolkinolide B. White needle-like crystals (CHCl3), m.p. 264-265 °C (m.p. 265 °C [31]), EI-MS m/z (%): 346 [M+] (3), 257 (12), 174 (13), 161 (14), 149 (18), 120 (24), 114 (32), 104 (38), 94 (43), 90 (66), 78 (63), 68 (65), 54 (82), 40 (100). C20H26O5. 1H-NMR (500 MHz) 4.70 (d, J = 12.0 Hz, 2H, H-17), 4.14 (s, 1H, H-14), 4.09 (s, 1H, H-11), 2.32 (s, 1H, H-9), 0.96 (s, 3H, H-18), 0.88 (s, 3H, H-19), 0.88 (s, 3H, H-20). 13C-NMR (125 MHz) 168.1 (C-16), 151.0 (C-13), 132.9 (C-15), 85.4 (C-12), 66.7 (C-8), 61.5 (C-11), 56.5 (C-17), 55.2 (C-14), 53.5 (C-5), 47.8 (C-9), 41.2 (C-3), 39.2 (C-10), 39.1 (C-1), 35.6 (C-7), 33.5 (C-4), 33.4 (C-18), 21.8 (C-19), 20.8 (C-6), 18.4 (C-2), 15.5 (C-20). The 1H- and 13C-NMR data were in agreement with the reported data [9,22,30].
Jolkinolide B. White needle-like crystals (CHCl3), m.p. 213-215 °C (m.p. 217 °C [28]), EI-MS m/z (%): 329 [M+] (4), 306 (14), 219 (15), 215 (25), 204 (26), 193 (30), 177 (36), 164 (38), 149 (40), 148 (98), 141 (72), 131 (37), 113 (35), 109 (94), 96 (100), 69 (43), 43 (37). C20H26O4. 1H-NMR (500 MHz) 4.07 (s, 1H, H-11), 3.71 (s, 1H, H-14), 2.32 (s, 1H, H-9), 2.11 (s, 3H, H-17), 0.97 (s, 3H, H-18), 0.88 (s, 3H, H-19), 0.85 (s, 3H, H-20). 13C-NMR (125 MHz) 169.0 (C-16), 148.6 (C-13), 130.2 (C-15), 85.2 (C-12), 66.0 (C-8), 60.9 (C-11), 55.3 (C-14), 53.4 (C-5), 48.0 (C-9), 41.2 (C-3), 39.2 (C-10), 39.1 (C-1), 35.6 (C-7), 33.5 (C-4), 33.4 (C-18), 21.8 (C-19), 20.8 (C-6), 18.4 (C-2), 15.4 (C-20), 8.75 (C-17). The 1H- and 13C-NMR data were in agreement with the reported data [32,35].
12-Deoxyphorbol 13-(9Z)-octadecenoate 20-acetate. White powder (CHCl3), EI-MS m/z (%): 654 [M+] (3), 337 (24), 311 (22), 313 (35), 239 (23), 237 (12), 149 (10), 134 (13), 123 (16), 108 (26), 97 (67), 69 (63), 55 (100). C40H62O7. 1H-NMR (500 MHz) 7.63 (1H, s, H-1), 5.73 (1H, d, J = 4.1Hz, H-7), 5.36 (2H, m, H-9¢ and H-10¢), 4.48 (2H, ABq, J = 12.3, 7.3 Hz, H-20), 3.30 (1H, brd, J = 2.2 Hz, H-10), 3.02 (1H, t, J = 5.1 Hz, H-8), 2.02 (3H, s, OAc-20). 13C-NMR (125 MHz) 209.1 (C-3), 161.4 (C-1), 135.0 (C-6), 133.8 (C-7), 132.8 (C-2), 75.9 (C-9), 73.6 (C-4), 69.4 (C-20), 63.2 (C-13), 55.7 (C-10), 39.4 (C-8), 38.9 (C-5), 36.3 (C-11), 32.4 (C-14), 31.9 (C-12), 23.2 (C-16), 22.6 (C-15), 18.5 (C-18), 15.3 (C-17), 10.0 (C-19), 175.9 (C-1¢), 34.2 (C-2¢), 24.9 (C-3¢), 28.9-29.8 (C-4¢-C-8¢ and C-12¢-C-15¢), 31.8 (C-16¢), 22.6 (C-17¢), 14.1 (C-18¢), 173.4 and 21.2 (OAc-20). The 1H- and 13C-NMR data were in agreement with the reported data [29].

3.7. Data analyses

Analysis of variance (ANOVA) and Tukey’s test were conducted by using SPSS 10 for Windows 98. Percentage was subjected to an arcsine square-root transformation before ANOVA and Tukey’s tests. The EC50 (the concentration needed to inhibit insect feeding by 50% relative to controls) was determined by linear regression (40).

4. Conclusions

Based on mass screening of medicinal herbs, the ethanol extract of E. fischeriana roots was found to possess feeding deterrent activity against the two grain storage insects, the red flour beetles (T. castaneum) and maize weevil (S. zeamais). Four feeding deterrents were isolated and identified from the ethanol extract of E. fischeriana roots by bioactivity-guided fractionation. The concentration used in this study to observe feeding deterrent effects was comparable with the commercially product toosendanin. These findings suggest that the ethanol extract of E. fischeriana roots and four isolated compound show potential for development as natural feeding deterrent fumigants for stored products.

Acknowledgements

This work was funded by National New-drug Innovation Project 2009ZX09501-014 and the Hi-Tech Research and Development of China 2006AA10A209. We thank Liu Q.R. from the College of Life Sciences, Beijing Normal University, Beijing 100875, for the identification of the investigated medicinal herb.

References and Notes

  1. Isman, M.B. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Ann. Rev. Entomol. 2006, 51, 45–66. [Google Scholar] [CrossRef]
  2. Isman, M.B. Perspective botanical insecticides: For richer, for poorer. Pest Manag. Sci. 2008, 64, 8–11. [Google Scholar] [CrossRef]
  3. The Pharmacopoeia Commission of PRChina. Pharmacopoeia of the People’s Republic of China; Guangdong Science and Technology Press: Guangzhou, China, 1995; Volume 1, p. 135.
  4. Liu, Z.L.; Ho, S.H. Bioactivity of the essential oil extracted from Evodia rutaecarpa Hook f. et Thomas against the grain storage insects, Sitophilus zeamais Motsch. and Tribolium castaneum (Herbst). J. Stored Prod. Res. 1999, 35, 317–328. [Google Scholar] [CrossRef]
  5. Magan, N.; Hope, R.; Cairns, V.; Aldred, D. Postharvest fungal ecology: impact of fungal growth and mycotoxin accumulation in stored grain. Eur. J. Plant Pathol. 2003, 109, 723–730. [Google Scholar] [CrossRef]
  6. Isman, M.B. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Ann. Rev. Entomol. 2006, 51, 45–66. [Google Scholar] [CrossRef]
  7. Jiangsu New Medical College. Encyclopedia of Chinese Medicinal Substances; Shanghai People’s Publisher: Shanghai, China, 1986; pp. 1898–1900.
  8. Qin, G.W.; Xu, R.S. Recent advances on bioactive natural products from Chinese medicinal plants. Med. Res. Rev. 1998, 18, 375–382. [Google Scholar] [CrossRef]
  9. Wu, Q.C.; Tang, Y.P.; Ding, A.W.; You, F.Q.; Zhang, L.; Duan, J.A. 13C-NMR data of three important diterpenes isolated from Euphorbia species. Molecules 2009, 14, 4454–4475. [Google Scholar] [CrossRef]
  10. Wang, B.; Zhao, H.M.; Lu, H. Euphorbia fischeriana Steud on killing Oncomelania snails and miracidiums of Schistosoma. Anhui Agr. Sci. Bull. 2010, 16, 46–48, (in Chinese with English abstract).. [Google Scholar]
  11. Yang, R.Z.; Tang, C.S. Plants used for pest control in China: a literature review. Econ. Bot. 1988, 42, 376–406. [Google Scholar] [CrossRef]
  12. Gu, Y.J.; Yang, L.F.; Zhang, L.J.; Li, X.H.; Liu, S.Q.; Cao, H. Studies of acaricidal activity of Euphorbia fisheriana against Tetranychus cinnabarinus (Boisduval). J. Shanxi Agr. Univ. 2007, 27, 394–396, (in Chinese with English abstract).. [Google Scholar]
  13. Liu, S.Q.; Gu, Y.J.; Wang, H.X.; Dong, H.L.; Shi, G.L.; Cao, H. Contact activities of extracts from different parts of Euphorbia fischeriana against Tetranychus cinnabarinus. Sci. Silvae Sin. 2010, 46, 111–115, (in Chinese with English abstract).. [Google Scholar]
  14. Xu, D.C.; Luo, C.G.; Chen, F.Z. Killing trials of pesticide of Radix Euphorbiae Fischerianae. China Beet Sugar 2004, 2, 5–7, (in Chinese with English abstract).. [Google Scholar]
  15. Uemura, D.; Sugiura, K.; Hirata, Y. O-Acetyl-N-(N'-benzoyl-L-phenylalanyl)-L-phenylalaninol. Isolation from Euphorbia fischeriana. Chem. Lett. 1975, 6, 537–538. [Google Scholar]
  16. Schroeder, G.; Rohmer, M.; Beck, J.P.; Anton, R. 7-Oxo-, 7α-hydroxy- and 7β-hydroxy-sterols from Euphorbia fischeriana. Phytochemistry 1980, 19, 2213–2215. [Google Scholar]
  17. Lee, S.H.; Tanaka, T.; Nonaka, G.; Nishioka, I.; Zhang, B. Allose gallates from Euphorbia fischeriana. Phytochemistry 1991, 30, 1251–1253. [Google Scholar] [CrossRef]
  18. Liu, W.Z.; Wu, X.Y.; Yang, G.J.; Ma, Q.G.; Zhou, T.X.; Tang, X.C.; Qin, G.W. 12-Deoxyphorbol esters from Euphorbia fischeriana. Chin. Chem. Lett. 1996, 7, 917–918. [Google Scholar]
  19. Liu, W.Z.; He, F.L.; Wu, X.Y.; Qin, G.W. Isolation and identification of terpenoides and anthraquinones from Euphorbia fischeriana. J. Chin. Med. Mater. 1997, 20, 351–353, (in Chinese with English abstract).. [Google Scholar]
  20. Ma, Q.G.; Liu, W.Z.; Wu, X.Y.; Zhou, T.X.; Qin, G.W. Chemical studies of Lang-Du, a traditional Chinese medicine. 1. Diterpenoids from Euphorbia fischeriana. Phytochemistry 1997, 44, 663–666. [Google Scholar] [CrossRef]
  21. Che, C.T.; Zhou, T.X.; Ma, Q.G.; Qin, G.W.; Williams, I.D.; Wu, H.M.; Shi, Z.S. Diterpenes and aromatic compounds from Euphorbia fischeriana. Phytochemistry 1999, 52, 117–121. [Google Scholar]
  22. Pei, Y.H.; Koike, K.; Han, B.; Jia, Z.H.; Nikaido, T. Fischeria A, a novel norditerpene lactone from Euphorbia fischeriana. Tetrahedron Lett. 1999, 40, 951–952. [Google Scholar] [CrossRef]
  23. Liu, W.Z.; He, F.L.; Ruan, Z.Y.; Gu, X.F.; Wu, X.Y.; Qin, G.W. Studies on chemical constituents from Euphorbia fischeriana Steud. Chin. J. Chin. Mater. Med. 2001, 26, 180–182, (in Chinese with English abstract).. [Google Scholar]
  24. Liu, W.Z.; Ma, Q.G.; Gu, X.F.; Qin, G.W. Isolation and identification of triterpenoids and acids from Euphorbia fischeriana. Nat. Prod. Res. Develop. 2003, 15, 396–397, (in Chinese with English abstract).. [Google Scholar]
  25. Zhou, T.X.; Bao, G.H.; Ma, Q.G.; Qin, G.W.; Che, C.T.; Yang, L.; Wang, C.; Zheng, Q.T. Langduin C, a novel dimeric diterpenoid from the roots of Euphorbia fischeriana. Tetrahedron Lett. 2003, 44, 135–137. [Google Scholar]
  26. Liu, W.Z.; Ma, Q.G.; Qin, G.W.; Wu, X.Y.; Gu, X.F. Chemical constituents of Euphorbia fischeriana. Chin. Trad. Herbal Drugs 2004, 35, 260, (in Chinese with English abstract).. [Google Scholar]
  27. Pan, Q.; Shi, M.F.; Min, Z.D. Studies on the 2D NMR spectra of jolkinolide diterpenoids from Euphorbia fischriana. J. Chin. Pharm. Univ. 2004, 35, 16–19, (in Chinese with English abstract).. [Google Scholar]
  28. Wang, Y.B.; Yao, G.M.; Wang, H.B.; Qin, G.W. A novel diterpenoid from Euphorbia fischeriana. Chem. Lett. 2005, 34, 1160–1161. [Google Scholar] [CrossRef]
  29. Wang, Y.B.; Huang, R.; Wang, H.B.; Jin, H.Z.; Lou, L.G.; Qin, G.W. Diterpenoids from the roots of Euphorbia fischeriana. J. Nat. Prod. 2006, 69, 967–970. [Google Scholar] [CrossRef]
  30. Wu, Q.C.; Tang, Y.P.; Ding, A.W.; You, F.Q.; Duan, J.A. Diterpenes and triterpenes from the roots of Euphorbia fischeriana. Chin. J. Nat. Med. 2010, 8, 101–103. [Google Scholar] [CrossRef]
  31. Liu, G.F.; Fu, Y.Q.; Yang, Z.Q.; Zhao, H.Q.; Fan, X.M. Isolation and identification of antitumor constituents of diterpenoids lactone in Euphorbia fischeriana Steud. Zhongyao Tongbao 1988, 13, 291–292, (in Chinese with English abstract).. [Google Scholar]
  32. Talapatra, S.K, Das; Talapatra, B. Sterostructures and molecular confomations of six diterpenes from Gelonium multiflorum. Phytochemistry 1989, 28, 1181–1183. [Google Scholar]
  33. Pei, Y.H.; Han, B.; Feng, B.M.; Hua, H.M. Studies on chemical constituents of Euphorbia fischeriana. Chin. Trad. Herbal Drugs 2002, 33, 591–592, (in Chinese with English abstract).. [Google Scholar]
  34. Liu, G.F.; Yang, S.S.; Yang, Z.Q.; Zhang, J.; Zhao, H.Q.; Fan, X.M. Isolation and identification of the lipophlic constituents from the root of Euphorbia fischeriana Steud. Zhongyao Tongbao 1987, 12, 484–486, (in Chinese with English abstract).. [Google Scholar]
  35. Uemura, D.; Hirata, Y. Two new diterpenoids, jolkinolides A and B, obtained from Euphorbia jolkini (Euphorbiaceae). Tetrahedron Lett. 1972, 15, 1387–1390. [Google Scholar] [CrossRef]
  36. Xie, Y.S.; Bodnaryk, R.P.; Fields, P.G. A rapid and simple flour-disk bioassay for testing substances active against stored-product insects. Can. Entomol. 1996, 128, 865–875. [Google Scholar] [CrossRef]
  37. Champagne, D.; Koul, O.; Isman, M.; Scudder, G.G.E.; Towers, G.H.N. Biological activity of limonoids from Rutales. Phytochemistry 1992, 31, 377–394. [Google Scholar]
  38. Liu, Z.L.; Xu, Y.J.; Wu, J.; Goh, S.H.; Ho, S.H. Feeding deterrents from Dictamnus dasycarpus Turcz against two stored-product insects. J. Agr. Food Chem. 2002, 50, 1447–1450. [Google Scholar] [CrossRef]
  39. Liu, Z.L.; Chu, S.S.; Jiang, G.H. Feeding deterrents from Zanthoxylum schinifolium against two stored-product insects. J. Agr. Food Chem. 2009, 57, 10130–10133. [Google Scholar] [CrossRef]
  40. Chen, C.C.; Chang, S.J.; Cheng, L.L.; Hou, R.F. Effects of chinaberry fruit extract on feeding, growth and fecundity of the diamondback moth, Plutella xylostella L. (Lep., Yponomeutidae). J. Appl. Entomol. 1996, 120, 341–345. [Google Scholar] [CrossRef]
  • Sample Availability: Samples of the crude extracts and pure compounds are available from the authors.

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MDPI and ACS Style

Geng, Z.F.; Liu, Z.L.; Wang, C.F.; Liu, Q.Z.; Shen, S.M.; Liu, Z.M.; Du, S.S.; Deng, Z.W. Feeding Deterrents against Two Grain Storage Insects from Euphorbia fischeriana. Molecules 2011, 16, 466-476. https://doi.org/10.3390/molecules16010466

AMA Style

Geng ZF, Liu ZL, Wang CF, Liu QZ, Shen SM, Liu ZM, Du SS, Deng ZW. Feeding Deterrents against Two Grain Storage Insects from Euphorbia fischeriana. Molecules. 2011; 16(1):466-476. https://doi.org/10.3390/molecules16010466

Chicago/Turabian Style

Geng, Zhu Feng, Zhi Long Liu, Cheng Fang Wang, Qi Zhi Liu, Sheng Min Shen, Zi Mu Liu, Shu Shan Du, and Zhi Wei Deng. 2011. "Feeding Deterrents against Two Grain Storage Insects from Euphorbia fischeriana" Molecules 16, no. 1: 466-476. https://doi.org/10.3390/molecules16010466

APA Style

Geng, Z. F., Liu, Z. L., Wang, C. F., Liu, Q. Z., Shen, S. M., Liu, Z. M., Du, S. S., & Deng, Z. W. (2011). Feeding Deterrents against Two Grain Storage Insects from Euphorbia fischeriana. Molecules, 16(1), 466-476. https://doi.org/10.3390/molecules16010466

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