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Keywords = triple quodruopole liquid chromatography mass spectrometry

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10 pages, 296 KiB  
Article
Quorum Sensing Activity in Pandoraea pnomenusa RB38
by Robson Ee, Yan-Lue Lim, Lin-Xin Kin, Wai-Fong Yin and Kok-Gan Chan
Sensors 2014, 14(6), 10177-10186; https://doi.org/10.3390/s140610177 - 10 Jun 2014
Cited by 5 | Viewed by 6303
Abstract
Strain RB38 was recovered from a former dumping area in Malaysia. MALDI-TOF mass spectrometry and genomic analysis identified strain RB-38 as Pandoraea pnomenusa. Various biosensors confirmed its quorum sensing properties. High resolution triple quadrupole liquid chromatography–mass spectrometry analysis was subsequently used to [...] Read more.
Strain RB38 was recovered from a former dumping area in Malaysia. MALDI-TOF mass spectrometry and genomic analysis identified strain RB-38 as Pandoraea pnomenusa. Various biosensors confirmed its quorum sensing properties. High resolution triple quadrupole liquid chromatography–mass spectrometry analysis was subsequently used to characterize the N-acyl homoserine lactone production profile of P. pnomenusa strain RB38, which validated that this isolate produced N-octanoyl homoserine lactone as a quorum sensing molecule. This is the first report of the production of N-octanoyl homoserine lactone by P. pnomenusa strain RB38. Full article
(This article belongs to the Section Biosensors)
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15 pages, 932 KiB  
Article
Quorum Sensing Activity of Aeromonas Caviae Strain YL12, A Bacterium Isolated from Compost
by Yan-Lue Lim, Robson Ee, Wai-Fong Yin and Kok-Gan Chan
Sensors 2014, 14(4), 7026-7040; https://doi.org/10.3390/s140407026 - 22 Apr 2014
Cited by 25 | Viewed by 8420
Abstract
Quorum sensing is a well-studied cell-to-cell communication method that involves a cell-density dependent regulation of genes expression mediated by signalling molecules. In this study, a bacterium isolated from a plant material compost pile was found to possess quorum sensing activity based on bioassay [...] Read more.
Quorum sensing is a well-studied cell-to-cell communication method that involves a cell-density dependent regulation of genes expression mediated by signalling molecules. In this study, a bacterium isolated from a plant material compost pile was found to possess quorum sensing activity based on bioassay screening. Isolate YL12 was identified using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry and molecular typing using rpoD gene which identified the isolate as Aeromonas caviae. High resolution tandem mass spectrometry was subsequently employed to identify the N-acyl homoserine lactone profile of Aeromonas caviae YL12 and confirmed that this isolate produced two short chain N-acyl homoserine lactones, namely C4-HSL and C6, and the production was observed to be cell density-dependent. Using the thin layer chromatography (TLC) bioassay, both AHLs were found to activate C. violaceum CV026, whereas only C6-HSL was revealed to induce bioluminescence expression of E. coli [pSB401]. The data presented in this study will be the leading steps in understanding the role of quorum sensing in Aeromonas caviae strain YL12. Full article
(This article belongs to the Section Biosensors)
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12 pages, 214 KiB  
Article
Pandoraea sp. RB-44, A Novel Quorum Sensing Soil Bacterium
by Robson Ee Han-Jen, Yin Wai-Fong and Chan Kok-Gan
Sensors 2013, 13(10), 14121-14132; https://doi.org/10.3390/s131014121 - 18 Oct 2013
Cited by 25 | Viewed by 7799
Abstract
Proteobacteria are known to communicate via signaling molecules and this process is known as quorum sensing. The most commonly studied quorum sensing molecules are N-acylhomoserine lactones (AHLs) that consists of a homoserine lactone moiety and an N-acyl side chain with various [...] Read more.
Proteobacteria are known to communicate via signaling molecules and this process is known as quorum sensing. The most commonly studied quorum sensing molecules are N-acylhomoserine lactones (AHLs) that consists of a homoserine lactone moiety and an N-acyl side chain with various chain lengths and degrees of saturation at the C-3 position. We have isolated a bacterium, RB-44, from a site which was formally a landfill dumping ground. Using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry analysis, this isolate was identified as a Pandoraea sp.which was then screened for AHL production using biosensors which indicated its quorum sensing properties. To identify the AHL profile of Pandoraea sp. RB-44, we used high resolution tandem mass spectrometry confirming that this isolate produced N-octanoylhomoserine lactone (C8-HSL). To the best of our knowledge, this is the first report that showed quorum sensing activity exhibited by Pandoraea sp. Our data add Pandoraea sp. to the growing number of bacteria that possess QS systems. Full article
(This article belongs to the Section Biosensors)
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