Search Results (5)

Defective phononic crystals (PnCs) have garnered significant attention for their ability to localize and amplify elastic wave energy within defect sites or to perform narrowband filtering at defect-band frequencies. The necessity for continuously tunable defect characteristics is driven by the variable excitation frequencies encountered in rotating machinery. Conventional tuning methodologies, including synthetic negative capacitors or inductors integrated with piezoelectric defects, are constrained to fixed, offline, and incremental adjustments. To address these limitations, the present study proposes an active feedback approach that facilitates online, wide-range steering of defect bands in a one-dimensional PnC. Each defect is equipped with a pair of piezoelectric sensors and actuators, governed by three independently tunable feedback gains: displacement, velocity, and acceleration. Real-time sensor signals are transmitted to a multivariable proportional controller, which dynamically modulates local electroelastic stiffness via the actuators. This results in continuous defect-band frequency shifts across the entire band gap, along with on-demand sensitivity modulation. The analytical model that incorporates these feedback gains has been demonstrated to achieve a level of agreement with COMSOL benchmarks that exceeds 99%, while concurrently reducing computation time from hours to seconds. Displacement- and acceleration-controlled gains yield predictable, monotonic up- or down-shifts in defect-band frequency, whereas the velocity-controlled gain permits sensitivity adjustment without frequency drifts. Furthermore, the combined-gain operation enables the concurrent tuning of both the center frequency and the filtering sensitivity, thereby facilitating an instantaneous remote reconfiguration of bandpass filters. This framework establishes a new class of agile, adaptive ultrasonic devices with applications in ultrasonic imaging, structural health monitoring, and prognostics and health management. Full article

In this paper, the design, simulations, and experimental results related to new analog circuits for voltage controlled synthetic inductors (VCSI) are presented. The new circuits based on a generalized impedance converter (GIC) are proposed for adaptive resonant piezoelectric shunt damping. The VCSIs are implemented using (1) an analog multiplier and (2) an operational transconductance amplifier (OTA) as voltage-controlled resistor. The simulation and experimental results for the new proposed VCSIs are presented and a comparative analysis follows. The proposed VCSIs work in a stable manner in parallel with negative impedance converters (NIC) to enhance structural damping in resonant piezoelectric resistive-inductive shunt applications. The behavior of the synthetic inductor is identical to a real inductor only in a specific frequency range and this situation can explain the reported spreading performance in the literature for resonant piezoelectric shunt damping. The simulation results are validated by a group of experimental investigations that confirm the improved stability and linearity of the new circuits proposed as VCSIs. Experimental results show that the VCSI based on an analog multiplier have an enhanced linearity in comparison with the OTA version in a limited voltage control range. Full article

Polyhydroxyalkanoates (PHAs), a family of natural biopolyesters, are widely used in many applications, especially in biomedicine. Since they are produced by a variety of microorganisms, they possess special properties that synthetic polyesters do not have. Their biocompatibility, biodegradability, and non-toxicity are the crucial properties that make these biologically produced thermoplastics and elastomers suitable for their applications as biomaterials. Bacterial or archaeal fermentation by the combination of different carbohydrates or by the addition of specific inductors allows the bioproduction of a great variety of members from the PHAs family with diverse material properties. Poly(3-hydroxybutyrate) (PHB) and its copolymers, such as poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHVB) or poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (PHB4HB), are the most frequently used PHAs in the field of biomedicine. PHAs have been used in implantology as sutures and valves, in tissue engineering as bone graft substitutes, cartilage, stents for nerve repair, and cardiovascular patches. Due to their good biodegradability in the body and their breakdown products being unhazardous, they have also been remarkably applied as drug carriers for delivery systems. As lately there has been considerable and growing interest in the use of PHAs as biomaterials and their application in the field of medicine, this review provides an insight into the most recent scientific studies and advances in PHAs exploitation in biomedicine. Full article

This paper considers the piezoelectric resistive and inductive RL shunt damping applied to rotationally periodic structures equipped with an array of regularly spaced piezoelectric patches. A method for simplifying the hardware, by reducing the size of the inductors and eliminating the use of synthetic inductors, is described. The paper compares two different ways of using the piezoelectric array: independent loops and parallel loops. It shows that, if a specific mode with n nodal diameters is targeted, mounting $4n$ piezoelectric patches in two parallel loops is as efficient as mounting them in $4n$ independent loops, while considerably reducing the demand on the inductors, L, (by $4n^2$). The method takes advantage of the mode shapes of rotationally periodic structures. The proposed method is validated numerically and experimentally on a rotationally periodic circular plate (nearly axisymmetric). The proposed technique is aimed at turbomachinery applications. Full article

Cloning and expression plasmids are the workhorses of modern molecular biology. Despite the pathway paved by synthetic biology, laboratories around the globe still relay on standard cloning techniques using plasmids with reporter proteins for positive clone selection, such as β-galactosidase alpha peptide complementation for blue/white screening or ccdB, which encodes for a toxic DNA gyrase. These reporters, when interrupted, serve as a positive clone detection system. In the present report, we show that molecular cloning plasmids bearing the coding sequence for a 25.4 kDa protein, AmilCP, encoded by a 685 bp gene, that is well expressed in Escherichia coli, render blue-purple colonies. Using this reporter protein, we developed and tested a cloning system based on the constitutive expression of the non-toxic AmilCP protein, that once interrupted, the loss of purple color serves to facilitate positive clone selection. The main advantage of this system is that is less expensive than other systems since media do not contain chromogenic markers such as X-gal, which is both expensive and cumbersome to prepare and use, or inductors such as IPTG. We also designed an inducible expression plasmid suitable for recombinant protein expression that also contains AmilCP cloning selection marker, a feature not commonly found in protein expression plasmids. The use of chromogenic reporters opens an important avenue for its application in other organisms besides E. coli for clone selection or even for mutant selection. Full article