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Okra (Abelmoschus esculentus) is an important agricultural crop of the Malvaceae family, cultivated across tropical, subtropical, and warm temperate regions. However, okra production faces numerous challenges from diverse pest species, including insects, nematodes, arachnids, and mites, that significantly reduce its yield. Major economic pests include the cotton aphid, cotton spotted bollworm, Egyptian bollworm, cotton mealybug, whitefly, cotton leafhopper, cotton bollworm, two-spotted spider mite, root-knot nematode, reniform nematode, cotton leaf roller, and flea beetle. Additionally, less prevalent pests such as the blister beetle, okra stem fly, red cotton bug, cotton seed bug, cotton looper, onion thrips, green plant bug, and lesion nematode are also described. This review also addresses fungal and oomycete diseases that present high risks to okra production, including damping-off, powdery mildew, Cercospora leaf spot, gray mold, Alternaria leaf spot and pod rot, Phyllosticta leaf spot, Fusarium wilt, Verticillium wilt, collar rot, stem canker, anthracnose, and fruit rot. In addition to these fungal diseases, okra is also severely affected by several viral diseases, with the most important being okra yellow vein mosaic disease, okra enation leaf curl disease, and okra mosaic disease, which can cause significant yield losses. Moreover, okra may also suffer from bacterial diseases, with bacterial leaf spot and blight, caused primarily by Pseudomonas syringae, being the most significant. This manuscript synthesizes the current knowledge on these pests. It outlines various management techniques and strategies to expand the knowledge base of farmers and researchers, highlighting the key role of integrated pest management (IPM). Full article

The spotted bollworm Earias vittella (Lepidoptera: Nolidae) is a polyphagous pest with enormous economic significance, primarily affecting cotton and okra. However, the lack of gene sequence information on this pest has a significant constraint on molecular investigations and the formulation of superior pest management strategies. An RNA-seq-based transcriptome study was conducted to alleviate such limitations, and de novo assembly was performed to obtain transcript sequences of this pest. Reference gene identification across E. vittella developmental stages and RNAi treatments were conducted using its sequence information, which resulted in identifying transcription elongation factor (TEF), V-type proton ATPase (V-ATPase), and Glyceraldehyde -3-phosphate dehydrogenase (GAPDH) as the most suitable reference genes for normalization in RT-qPCR-based gene expression studies. The present study also identified important developmental, RNAi pathway, and RNAi target genes and performed life-stage developmental expression analysis using RT-qPCR to select the optimal targets for RNAi. We found that naked dsRNA degradation in the E. vittella hemolymph is the primary reason for poor RNAi. A total of six genes including Juvenile hormone methyl transferase (JHAMT), Chitin synthase (CHS), Aminopeptidase (AMN), Cadherin (CAD), Alpha-amylase (AMY), and V-type proton ATPase (V-ATPase) were selected and knocked down significantly with three different nanoparticles encapsulated dsRNA conjugates, i.e., Chitosan-dsRNA, carbon quantum dots-dsRNA (CQD-dsRNA), and Lipofectamine-dsRNA conjugate. These results demonstrate that feeding nanoparticle-shielded dsRNA silences target genes and suggests that nanoparticle-based RNAi can efficiently manage this pest. Full article

Earias vittella Fabricius is a potential cotton and okra pest in South Asia. The realized heritability, risk assessment, and inheritance mode of Bacillus thuringiensis Kurstaki (Btk) resistance were determined in the Dipel-selected (DIPEL-SEL) E. vittella. The DIPEL-SEL strain had a 127.56-fold rise in Dipel resistance after nine generations compared to the laboratory reference strain (LAB-PK). The overlapping of 95% fiducial limits in the median lethal concentrations (LC₅₀s) of the F₁ (DIPEL-SEL♂ × LAB-PK♀) and F₁^ǂ (DIPEL-SEL♀ × LAB-PK♂) suggested a lack of sex linkage and an autosomal Dipel resistance. The dominance (D_LC) values for the F₁ (0.86) and F₁^ǂ (0.94) indicated incompletely dominant resistance to Dipel. Backcrossing of the F₁♀ × Lab-PK♂ revealed a polygenic response of resistance to Dipel. The realized heritability estimation (h²) of resistance to Dipel was 0.19. With 20% to 90% selection mortality, the generations required for a tenfold increase in LC₅₀ of Dipel were 4.7–22.8, 3.1–14.9, and 2.3–11.1 at h² of 0.19, 0.29, and 0.39, respectively, and a constant slope of 1.56. At slope values of 2.56 and 3.56 with a constant h² = 0.19, 7.7–37.4 and 10.6–52.0 generations were needed to increase the tenfold LC₅₀ of Dipel in the DIPEL-SEL E. vittella. It is concluded that the DIPEL-SEL E. vittella has an autosomal, incompletely dominant, and polygenic nature of resistance. The h² of 0.19 suggested that a high proportion of phenotypic variation for the Dipel resistance in E. vittella was heritable genetic variation. The present results will support the creation of an effective and suitable resistance management plan for better control of E. vittella. Full article