Search Results (6)

This study reports findings from a nationwide replication and the second randomized controlled trial (RCT) of Strategic and Interactive Writing Instruction (SIWI), a linguistically responsive framework for teaching writing to deaf students. A total of 50 teachers and their 294 students in grades 3–6 were randomly assigned to either SIWI or business-as-usual (BAU) instruction. Writing outcomes were assessed with trait-based rubrics and the Structured Analysis of Written Language (SAWL) in two genres (recount and information report), along with the Woodcock–Johnson IV broad written language composite and genre-specific motivation surveys administered at the beginning and end of the school year. Students receiving SIWI outperformed peers in the BAU group on writing traits across both genres, with effect sizes ranging from moderately large (d = 0.70) for informational reports to very large (d = 1.11) for recounts. On the SAWL, SIWI students demonstrated significantly greater gains in grammatical clarity on recount writing, as measured by the word efficiency ratio, with a moderate effect size (d = 0.64), although this effect was not observed for information reports. Students in the treatment group also reported significantly higher motivation for both genres. Unlike the prior RCT, no statistically significant differences emerged on the broad written language measure (d = 0.27). This may reflect spurious findings in the previous study or limitations in this study caused by the COVID-19 pandemic. Nonetheless, the effect size observed suggests some practical importance that warrants further investigation. Findings provide robust evidence that SIWI positively impacts deaf elementary students’ writing development and motivation, particularly for recount genres, while underscoring the importance of replication for understanding the generalizability of intervention effects. Full article

The increasing prevalence of diabetic reproductive complications has prompted the development of innovative animal models. The use of the silkworm Bombyx mori as a model for diabetic reproductive damage shows potential as a valuable research tool. This study employed silkworms as a novel model to investigate diabetic reproductive damage. The silkworms were fed a high-glucose diet containing 10% glucose to induce a diabetic model. Subsequently, the study concentrated on assessing the influence of diabetes on the reproductive system of male silkworms. The results indicate that diabetes resulted in reduced luteinizing hormone (LH) and testosterone (T) levels, as well as elevated triglyceride (TG) levels in male silkworms. Moreover, diabetes mellitus was associated with pathological testicular damage in male silkworms, accompanied by decreased glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) levels, along with increased malondialdehyde (MDA) levels in the testis. Additionally, diabetes mellitus reduced the expression of siwi1 and siwi2 genes in the testis of male silkworms. Overall, these results support using silkworms as a valuable model for studying diabetic reproductive damage. Full article

The Colorado potato beetle (Leptinotarsa decemlineata (Say)) can cause extensive damage to agricultural crops worldwide and is a significant insect pest. This insect is notorious for its ability to evade various strategies deployed to control its spread and is known for its relative ease in developing resistance against different insecticides. Various molecular levers are leveraged by L. decemlineata for this resistance to occur, and a complete picture of the genes involved in this process is lacking. While small non-coding RNAs, including miRNAs, are differentially expressed in insects exposed to insecticides, levels of transcript coding for proteins underlying their synthesis remain to be characterized fully. The overarching objective of this work aims to fill that gap by assessing the expression of such targets in L. decemlineata exposed to cyantraniliprole and thiamethoxam. The expression status of Ago1, Ago2, Ago3, Dcr2a, Dcr2b, Expo-5, Siwi-1 and Siwi-2 transcripts were quantified via qRT-PCR in adult L. decemlineata treated with low and high doses of these compounds for different lengths of time. Variation in Ago1 and Dcr2b expression was notably observed in L. decemlineata exposed to cyantraniliprole, while thiamethoxam exposure was associated with the modulation of Dcr2a and Siwi-1 transcript levels. The down-regulation of Ago1 expression in L. decemlineata using dsRNA, followed by cyantraniliprole treatment, was associated with a reduction in the survival of insects with reduced Ago1 transcript expression. Overall, this work presents the insecticide-mediated modulation of transcripts associated with small non-coding RNA processing and showcases Ago1 as a target to further investigate its relevance in cyantraniliprole response. Full article

Date palm (Phoenix dactylifera L.) fruits contain high concentrations of phenolic compounds, particularly flavonoids and other micronutrients, which impact human health due to their potent antioxidant, anti-inflammatory, and anticancer characteristics. In the present study, the effect of ethyl acetate, hydroethanol, hydromethanol, and aqueous extract from three date palm varieties (i.e., Ajwa, Siwi, and Sukkari) on phytochemical profiles and antioxidant and anticancer activities was investigated. Fruit extracts were screened for their antioxidant activity using the DPPH· method. Phenolic constituents were quantified and identified using HPLC-DAD. Extracts (ethyl acetate, hydroethanol, and hydromethanol) were assessed for cytotoxicity on nine human cancer cell lines, i.e., MG-63, HCT116, MCF7, MDA-MB-231, HEPG2, HUH7, A549, H460, and HFB4, using the sulphorhodamine-B (SRB) assay. Results showed that the ethyl acetate extract of the Sukkari fruits has the greatest antioxidant potential with an IC₅₀ value of 132.4 ± 0.3 μg·mL⁻¹, while the aqueous extract of Ajwa date fruits exhibited the lowest antioxidant effect with an IC₅₀ value of 867.1 ± 0.3 μg·mL⁻¹. The extracts exhibited potent to moderate anticancer activities against the investigated cancer cell line in a source-dependent manner. Methanol extract of Siwi fruits exhibited the most potent anticancer activity (IC₅₀ = 99 ± 1.6 µg·mL⁻¹), followed by the same extract of Sukkari fruits with an IC₅₀ value of 119 ± 3.5 µg·mL⁻¹ against the cell line of human breast cancer (MDA-MB-231). Additionally, principal component analysis (PCA) was investigated to determine the relationship among the investigated traits and treatments. Our findings reveal that date palm fruit-derived extracts are excellent sources of biologically active constituents and substantiate their potential use in new anticancer strategies from natural resources. Full article

A field experiment was carried out at Al-Bahariya Oasis, Giza, Egypt, during three successive seasons to find out the effect of application of bentonite (BN) as a natural clay deposit at either 6 or 12 kg/palm tree and humic substances (HS) as organic amendment at either 0.75 or 1.0 L/palm tree incorporated with Bacillus polymyxa (BP) as a biofertilizers at 14 and 28 mL/L rates on growth, yield, and fruit weight of 10-year-old Siwi date palm cv. (Phoenix dectylifera L.). Siwi trees were cultivated in sandy texture soil at a distance of 8 × 8 m and were irrigated with 100%, 85%, and 70% of ET crop. Effects of the previous treatments on growth, date palm crop, soil properties, water relations, water-use efficiency (WUE), and economic return were also studied. The obtained results showed that the mean values of leaf length, leaflet length and width, fruit set%, bunch weight, yield/palm, yield/fed, fruit weight, flesh weight, and fruit pulp weight were increased in response to the different individual and combined treatments used in this study with various significance levels compared to the control treatment means in the studied three seasons. However, the superiority was for combining between amending the sandy soil with either low concentrations of BN (6 kg/tree) + HS (0.75 Liter/tree) + BP (14 mL/L) or high concentrations of BN (12 kg/tree) + HS (1.0 L/tree) + BP (28 mL/L) and irrigation with 85% of ETc water level, as such combinations attained the highest values in most of the mentioned values over both the sole and combined treatments in the studied three seasons. Furthermore, the results indicated that the highest monthly ETc values occurred during June and July months, while the lowest values occurred during December and January months. Additionally, water productivity (WP) increased considerably by reduction of water quantity (70% ETc) associated with soil conditioner treatment (BN.12 kg +HS 1 L + BP. 28 mL/L), and values were 2.17, 2.25, and 2.27 kg fruit/m³ of water irrigation during the growing seasons, respectively. The highest net return was attributed to irrigation with 85% of ETc water level along with the application of soil conditioners at high rates. Accordingly, it is advisable to apply the soil conditioners of bentonite (at 6 or 12 kg/tree) and humic substances (at either 0.75 or 1.0 L/tree) with B. polymyxa (at either 14 or 28 mL/L) plus irrigation with either 85% or 70% of ETc water level to obtain the best growth, highest yield, (WP), and gross return from var. Siwi date palm grown under an oasis agro-system. Full article

Insect antiviral immunity primarily relies on RNAi mechanisms. While a key role of small interfering (si)RNAs and AGO proteins has been well established in this regard, the situation for PIWI proteins and PIWI-interacting (pi)RNAs is not as clear. In the present study, we investigate whether PIWI proteins and viral piRNAs are involved in the immunity against single-stranded RNA viruses in lepidopteran cells, where two PIWIs are identified (Siwi and Ago3). Via loss- and gain-of-function studies in Bombyx mori BmN4 cells and in Trichoplusia ni High Five cells, we demonstrated an antiviral role of Siwi and Ago3. However, small RNA analysis suggests that viral piRNAs can be absent in these lepidopteran cells. Together with the current literature, our results support a functional diversification of PIWI proteins in insects. Full article