Open AccessArticle
Mutation Profiles of Ovarian Seromucinous Borderline Tumors in Japanese Patients
by
Hiroki Sasamori, Kentaro Nakayama, Sultana Razia, Hitomi Yamashita, Tomoka Ishibashi, Masako Ishikawa, Seiya Sato, Satoru Nakayama, Yoshiro Otsuki, Ritsuto Fujiwaki, Noriyoshi Ishikawa and Satoru Kyo
Cited by 4 | Viewed by 3208
Abstract
Ovarian seromucinous tumors (SMBTs) are relatively rare, and their carcinogenesis is largely unknown. In this study, the molecular features of SMBTs in Japan are assessed. DNA was extracted from microdissected paraffin-embedded sections from 23 SMBT cases. Genetic mutations (
KRAS,
BRAF,
[...] Read more.
Ovarian seromucinous tumors (SMBTs) are relatively rare, and their carcinogenesis is largely unknown. In this study, the molecular features of SMBTs in Japan are assessed. DNA was extracted from microdissected paraffin-embedded sections from 23 SMBT cases. Genetic mutations (
KRAS,
BRAF,
PIK3CA, and
ERBB2) were evaluated using Sanger sequencing. Immunohistochemistry for p53, ARID1A, and PTEN was also performed as a surrogate for the loss of functional mutations in these tumor suppressor genes. The prevalence of
KRAS, BRAF, PIK3CA, and
ERBB2 mutations was 4.3% (1/23), 8.6% (2/23), 8.6% (2/23), and 17.3% (4/23), respectively. Overexpression or loss of p53 expression occurred in 26% (6/23), loss of ARID1A expression in 4.3% (1/23), and none of the cases showed expression of PTEN loss. These findings suggest that
KRAS/BRAF/PIK3CA and
PTEN mutations are rare carcinogenic events in SMBTs. The high frequency of positive p53 staining and a low frequency of loss of ARID1A staining suggests that SMBT carcinogenesis may be related to the alteration of
p53 rather than that of
ARID1A.
ERBB2 oncogenic mutations may play an important role in the tumorigenesis of Japanese SMBTs.
Full article
►▼
Show Figures
