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Keywords = optically-encoded bead

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17 pages, 867 KB  
Review
Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads
by Bong-Hyun Jun, Homan Kang, Yoon-Sik Lee and Dae Hong Jeong
Molecules 2012, 17(3), 2474-2490; https://doi.org/10.3390/molecules17032474 - 1 Mar 2012
Cited by 78 | Viewed by 13159
Abstract
Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over [...] Read more.
Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding. Full article
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