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Keywords = multimeric thymosin

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17 pages, 7871 KB  
Article
Cloning, Expression and Effects of P. americana Thymosin on Wound Healing
by Jie Jing, Xiaohong Sun, Chuang Zhou, Yifan Zhang, Yongmei Shen, Xiaomao Zeng, Bisong Yue and Xiuyue Zhang
Int. J. Mol. Sci. 2019, 20(19), 4932; https://doi.org/10.3390/ijms20194932 - 5 Oct 2019
Cited by 25 | Viewed by 4957
Abstract
The American cockroach (Periplaneta americana) is a medicinal insect. Its extract is used clinically to promote wound healing and tissue regeneration, but the effective medicinal components and mechanisms are not yet clear. It has been reported that human thymosin beta 4 [...] Read more.
The American cockroach (Periplaneta americana) is a medicinal insect. Its extract is used clinically to promote wound healing and tissue regeneration, but the effective medicinal components and mechanisms are not yet clear. It has been reported that human thymosin beta 4 (Tβ4) may accelerate skin wound healing, however, the role of P. americana thymosin (Pa-THYs) is still poorly understood. In the present study, we identify and analyze the DNA sequences of Pa-THYs by bioinformatics analysis. Then we clone, express, and purify the Pa-THYs proteins and evaluate the activity of recombinant Pa-THYs proteins by cell migration and proliferation assays in NIH/3T3 cells. To elucidate the role of Pa-THYs in wound healing, a mouse model is established, and we evaluate wound contraction, histopathological parameters, and the expressions of several key growth factors after Pa-THYs treatment. Our results showed that three THY variants were formed by skipping splicing of exons. Pa-THYs could promote fibroblast migration, but have no effect on fibroblast proliferation. In wound repair, Pa-THYs proteins could effectively promote wound healing through stimulating dermal tissue regeneration, angiogenesis, and collagen deposition. On the molecular mechanism, Pa-THYs also stimulated the expression of several key growth factors to promote wound healing. The data suggest that Pa-THYs could be a potential drug for promoting wound repair. Full article
(This article belongs to the Special Issue Wound Repair and Regeneration: Mechanisms, Signaling)
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