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Keywords = immuno-dipstick

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12 pages, 8702 KiB  
Article
High-Density Gold Nanoparticles Implanted on Mg/Fe LDH Nanoflowers Assisted Lateral Flow Immuno-Dipstick Assay for Visual Detection of Human Epididymal Protein 4
by Hao Liu, Mei-Xia Wu and Shou-Nian Ding
Biosensors 2022, 12(10), 797; https://doi.org/10.3390/bios12100797 - 27 Sep 2022
Cited by 5 | Viewed by 2558
Abstract
The timelier and more accurate the diagnosis of the disease, the higher the patient’s survival rate. Human epididymal protein 4 (HE4) has great significance as a biomarker of concern for reflecting ovarian cancer. Herein, we prepared a novel optical label that can be [...] Read more.
The timelier and more accurate the diagnosis of the disease, the higher the patient’s survival rate. Human epididymal protein 4 (HE4) has great significance as a biomarker of concern for reflecting ovarian cancer. Herein, we prepared a novel optical label that can be used in lateral-flow immuno-dipstick assay (LFIA) for sensitive visual detection of HE4 by implanting hydrophobic gold nanoparticles (Au NPs) at high density in Mg/Fe LDH nanoflowers (MF NFs). MF NFs with large specific surface area, high porosity, abundant active binding sites, and stable structure were employed for the first time as templates to directly anchor Au NPs in the organic phase. After simple modification with an optimized amount of branched polyethyleneimine, not only could MF@Au NFs be dispersed in the aqueous phase, but also amino functional groups were introduced on its surface to facilitate subsequent antibody coupling steps. The limit of detection reaches 50 pM with a detection range of 50 to 1000 pM. This work initially explored how MF NFs can be used to load signal labels with ideal stability and signal amplification capabilities, which greatly improves the practicability of LFIA and highlights its important role in the field of rapid diagnostics. Full article
(This article belongs to the Special Issue Biosensing and Diagnosis)
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15 pages, 2884 KiB  
Article
Immuno-Dipstick for Colletotrichum gloeosporioides Detection: Towards On-Farm Application
by Fifame Auriane Oussou-Azo, Taiki Futagami and Mun’delanji Catherine M. Vestergaard
Biosensors 2022, 12(2), 49; https://doi.org/10.3390/bios12020049 - 18 Jan 2022
Cited by 3 | Viewed by 4186
Abstract
Early and quick detection of pathogens are crucial for managing the spread of infections in the biomedical, biosafety, food, and agricultural fields. While molecular diagnostics can offer the specificity and reliability in acute infectious diseases, detection of pathogens is often slowed down by [...] Read more.
Early and quick detection of pathogens are crucial for managing the spread of infections in the biomedical, biosafety, food, and agricultural fields. While molecular diagnostics can offer the specificity and reliability in acute infectious diseases, detection of pathogens is often slowed down by the current benchtop molecular diagnoses, which are time consuming, labor intensive, and lack the mobility for application at the point-of-need. In this work, we developed a complete on-farm use detection protocol for the plant-devastating anthracnose agent: Colletotrichum gloeosporioides. Our methods combined a simplified DNA extraction on paper that is compatible with loop-mediated isothermal amplification (LAMP), coupled with paper-based immunoassay lateral flow sensing. Our results offer simple, quick, easy, and a minimally instrumented toolkit for Colletotrichum gloeosporioides detection. This scalable and adaptable platform is a valuable alternative to traditional sensing systems towards on-the-go pathogen detection in food and agriculture, biomedical, and other fields. Full article
(This article belongs to the Special Issue Microbial Toxins and Pathogen Biodetection)
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