Search Results (9)

Alginate is a naturally occurring polymer derived from brown algae biomass, which has numerous applications in various fields. Chemical modification of alginate is widely used to improve alginate’s physicochemical properties and provide new potential for multiple applications. In this article, we modified alginate with L-DOPA, using periodate oxidation and reductive amination, to obtain more suitable biopolymer for biocatalyst immobilization and hydrogel formation. Obtained modified alginate was used for the immobilization of laccase on cell walls. For this purpose, laccase from Streptomyces cyaneus was expressed on the surface of Saccharomyces cerevisiae EBY100 cells. The obtained cell wall laccase was immobilized within L-DOPA-alginate beads by crosslinking the L-DOPA-alginate with calcium ions and laccase. The effect of additional crosslinking of beads by green light-induced photopolymerization with eosin Y was investigated. The immobilized laccase systems were used for dye decolorization and investigated in multiple treatment processes. Beads with L-DOPA-alginate with a higher degree of modification (5.0 mol%) showed higher enzymatic activity and better decolorization efficiency than those with a lower degree of modification (2.5 mol%). Obtained immobilized biocatalysts are suitable for decolorizing dye Evans Blue due to their high efficiency and reusability. Full article

Immense use of fossil fuels leads to various environmental issues, including greenhouse gas emissions, reduced oil reserves, increased energy costs, global climate changes, etc. These challenges can be tackled by using alternative renewable fuels such as biodiesel. Many studies reported that biodiesel production from microalgae biomass is an environment-friendly and energy-efficient approach, with significantly improved fuel quality in terms of density, calorific value and viscosity. Biodiesel is produced using the transesterification process and the most sustainable method is utilizing enzymes for transesterification. Lipase is an enzyme with excellent catalytic activity, specificity, enantio-selectivity, compatibility and stability and hence it is applied in microalgae biodiesel production. But, difficulty in enzymatic recovery, high enzyme cost and minimal reaction rate are some of its drawbacks that have to be addressed. In this aspect, the nanotechnological approach of lipase immobilization in producing microalgae biodiesel is a promising way to increase production yield and it is due to the adsorption efficiency, economic benefit, recyclability, crystallinity, durability, stability, environmental friendliness and catalytic performance of the bio-nanoparticles used. Through increasing post-harvest biomass yield, absorption of CO₂ and photosynthesis in the photobioreactor, the use of nanoparticle immobilized lipase during the generation of biodiesel from microalgae has the potential to also remove feedstock availability constraints. This review article discusses the production of microalgae biodiesel, and effect of nanoparticles and immobilized lipase nanoparticles on biodiesel production. The advantages of using lipase nanoparticles and the challenges in introducing the immobilized lipase on nanoparticles in large-scale microalgae biodiesel production are also discussed. Reducing the water and land use, energy and nutrient footprints of integrated algae-based operations must be the main goal of larger-scale experiments as well as ongoing research and development in order to expedite the adoption of microalgae-based biodiesel production. Also, the cost-effectiveness and large-scale availability of nanoparticles and the impact of lipase nanoparticles on engine performance should be analyzed for commercialization of microalgae biodiesel. Full article

Light-emitting diodes are increasingly used as artificial light sources in Haematococcus pluvialis cultivation due to the fact of their energy advantages. The immobilized cultivation of H. pluvialis in pilot-scale angled twin-layer porous substrate photobioreactors (TL-PSBRs) was initially performed with a 14/10 h light/dark cycle and showed relatively low biomass growth and astaxanthin accumulation. In this study, the illumination time with red and blue LEDs at a light intensity of 120 µmol photons m⁻² s⁻¹ was increased to 16–24 h per day. With a light/dark cycle of 22/2 h, the biomass productivity of the algae was 7.5 g m⁻² day⁻¹, 2.4 times higher than in the 14/10 h cycle. The percentage of astaxanthin in the dry biomass was 2%, and the total amount of astaxanthin was 1.7 g m⁻². Along with the increase in light duration, adding 10 or 20 mM NaHCO₃ to the BG11-H culture medium over ten days of cultivation in angled TL-PSBRs did not increase the total amount of astaxanthin compared with only CO₂ addition at a flow rate of 3.6 mg min⁻¹ to the culture medium. Adding NaHCO₃ with a 30–80 mM concentration inhibited algal growth and astaxanthin accumulation. However, adding 10–40 mM NaHCO₃ caused algal cells to accumulate astaxanthin at a high percentage in dry weight after the first four days in TL-PSBRs. Full article

The production of poly(hydroxylalkanoates) (PHA) is limited by the high cost of the feedstock since various biomass wastes look attractive as possible sources for polymer production. The originality of this present study is in the biotransformation of mixed Chlorella-based substrates into PHAs. The synthetic potential of Cupriavidus necator B8619 cells was studied during the bioconversion of algae biomass in mixtures with spent immobilized mycelium of different fungi (genus Rhizopus and Aspergillus) into PHAs. The biomass of both microalgae Chlorella and fungus cells was accumulated due to the use of the microorganisms in the processes of food wastewater treatment. The biosorption of Chlorella cells by fungal mycelium was carried out to obtain mixed biomass samples (the best ratio of “microalgae:fungi” was 2:1) to convert them by C. necator B8619 into the PHA. The influence of conditions used for the pretreatment of microalgae and mixed types of biomass on their conversion to PHA was estimated. It was found that the maximum yield of reducing sugars (39.4 ± 1.8 g/L) can be obtained from the mechanical destruction of cells by using further enzymatic hydrolysis. The effective use of the enzymatic complex was revealed for the hydrolytic disintegration of treated biomass. The rate of the conversion of mixed substrates into the biopolymer (440 ± 13 mg/L/h) appeared significantly higher compared to similar known examples of complex substrates used for C. necator cells. Full article

In the field of environmental science and engineering, microorganisms, enzymes and algae are promising biomass materials that can effectively degrade pollutants. However, problems such as poor environmental adaptability, recycling difficulties, and secondary pollution exist in the practical application of non-immobilized biomass materials. Biomass immobilization is a novel environmental remediation technology that can effectively solve these problems. Compared with non-immobilized biomass, immobilized biomass materials have the advantages of reusability and stability in terms of pH, temperature, handling, and storage. Many researchers have studied immobilization technology (i.e., methods, carriers, and biomass types) and its applications for removing refractory organic pollutants. Based on this, this paper reviews biomass immobilization technology, outlines the mechanisms and factors affecting the removal of refractory organic pollutants, and introduces the application of immobilized biomass materials as fillers for reactors in water purification. This review provides some practical references for the preparation and application of immobilized biomass materials and promotes further research and development to expand the application range of this material for water purification. Full article

Microalgae-mediated CO₂ sequestration has been a subject of numerous research works and has become one of the most promising strategies to mitigate carbon dioxide emissions. However, feeding flue and exhaust gas into algae-based systems has been shown to destroy chloroplasts, as well as disrupt photosynthesis and other metabolic processes in microalgae, which directly limits CO₂ uptake. CO₂ biosequestration in existing photobioreactors (PBRs) is also limited by the low biomass concentration in the growth medium. Therefore, there is a real need to seek alternative solutions that would be competitive in terms of performance and cost-effectiveness. The present paper reports the results of experiments aimed to develop an innovative trickle bed reactor that uses immobilized algae to capture CO₂ from flue and exhaust gas (IMC-CO₂PBR). In the experiment, ambient air enriched with technical-grade CO₂ to a CO₂ concentration of 25% v/v was used. The microalgae immobilization technology employed in the experiment produced biomass yields approximating 100 g DM/dm³. A relationship was found between CO₂ removal rates and gas volume flux: almost 40% of CO₂ was removed at a feed of 25 dm³ of gas per hour, whereas in the 200 dm³/h group, the removal efficiency amounted to 5.9%. The work includes a determination of basic process parameters, presentation of a developed functional model and optimized lighting system, proposals for components to be used in the system, and recommendations for an automation and control system for a full-scale implementation. Full article

In the production of astaxanthin from Haematococcus pluvialis, the process of growing algal biomass in the vegetative green stage is an indispensable step in both suspended and immobilized cultivations. The green algal biomass is usually cultured in a suspension under a low light intensity. However, for astaxanthin accumulation, the microalgae need to be centrifuged and transferred to a new medium or culture system, a significant difficulty when upscaling astaxanthin production. In this research, a small-scale angled twin-layer porous substrate photobioreactor (TL-PSBR) was used to cultivate green stage biomass of H. pluvialis. Under low light intensities of 20–80 µmol photons m⁻²·s⁻¹, algae in the biofilm consisted exclusively of non-motile vegetative cells (green palmella cells) after ten days of culturing. The optimal initial biomass density was 6.5 g·m⁻², and the dry biomass productivity at a light intensity of 80 µmol photons m⁻²·s⁻¹ was 6.5 g·m⁻²·d⁻¹. The green stage biomass of H. pluvialis created in this small-scale angled TL-PSBR can be easily harvested and directly used as the source of material for the inoculation of a pilot-scale TL-PSBR for the production of astaxanthin. Full article

Microalgae has received overwhelming attention worldwide as a sustainable source for energy generation. However, the production of biofuel from microalgae biomass consists of several steps, of which lipid extraction is the most important one. Because of the nature of feedstock, extraction needs special attention. Three different methods were studied to extract algal oil from two different algae variant, Chlorella sp. and Spirulina sp. The highest percentage oil yield was obtained by ultrasonication (9.4% for Chlorella sp., 6.6% for Spirulina sp.) followed by the Soxhlet and solvent extraction processes. Ultrasonication and Soxhlet extraction processes were further optimized to maximize oil extraction as solvent extraction was not effective in extracting lipid. For ultrasonication, an amplitude of 90% recorded the highest percentage yield of oil for Spirulina sp. and a 70% amplitude recorded the highest percentage yield of oil for Chlorella sp. On the other hand, for Soxhlet extraction, a combination of chloroform, hexane, and methanol at a 1:1:1 ratio resulted in the highest yield of algal oil. Afterward, the crude algae oil from the ultrasonication process was transesterified for 5 h using an immobilized lipase (Novozyme 435) at 40 °C to convert triglycerides into fatty acid methyl ester and glycerol. Thus, ultrasonic-assisted lipid extraction was successful in producing biodiesel from both the species. Full article

The microalga Haematococcus pluvialis is mainly cultivated in suspended systems for astaxanthin production. Immobilized cultivation on a Twin-Layer porous substrate photobioreactor (TL-PSBR) has recently shown promise as an alternative approach. In Vietnam, a TL-PSBR was constructed as a low-angle (15 °) horizontal system to study the cultivation of H. pluvialis for astaxanthin production. In this study, the biomass and astaxanthin productivities and astaxanthin content in the dry biomass were determined using different initial biomass (inoculum) densities (from 2.5 to 10 g dry weight m⁻²), different storage times of the initial biomass at 4 °C (24, 72, 120 and 168 h) and different light intensities (300–1000 µmol photons m⁻² s⁻¹). The optimal initial biomass density at light intensities between 400–600 µmol photons⁻² s⁻¹ was 5–7.5 g m⁻². Algae stored for 24 h after harvest from suspension for immobilization on the TL-PSBR yielded the highest biomass and astaxanthin productivities, 8.7 g m⁻² d⁻¹ and 170 mg m⁻² d⁻¹, respectively; longer storage periods decreased productivity. Biomass and astaxanthin productivities were largely independent of light intensity between 300–1000 µmol photons m⁻² s⁻¹ but the efficiency of light use per mole photons was highest between 300–500 µmol photons m⁻² s⁻¹. The astaxanthin content in the dry biomass varied between 2–3% (w/w). Efficient supply of CO₂ to the culture medium remains a task for future improvements of angled TL-PSBRs. Full article