Search Results (2)

A new comprehensive analytical approach based on single-particle inductively coupled plasma-sector field mass spectrometry (spICP-SFMS) and electrical asymmetric-flow field-flow-fractionation combined with multi-angle light scattering detection (EAF4-MALS) has been examined for the characterization of galactosamine-terminated poly(N-hydroxyethyl acrylamide)-coated gold nanorods (GNRs) in two different degrees of polymerization (DP) by tuning the feed ratio (short: DP 35; long: DP 60). spICP-SFMS provided information on the particle number concentration, size and size distribution of the GNRs, and was found to be useful as an orthogonal method for fast characterization of GNRs. Glycoconjugated GNRs were separated and characterized via EAF4-MALS in terms of their size and charge and compared to the bare GNRs. In contrast to spICP-SFMS, EAF4-MALS was also able of providing an estimate of the thickness of the glycopolymer coating on the GNRs surface. Full article

As the diversity of nanomaterials is wide and their size can vary by 2 orders of magnitude (1−100 nm), the development of new and advanced analytical tools for their in-depth characterization is of paramount importance, allowing a fundamental understanding of their structure, further alteration and degree of chemical surface functionalization. Herein, we present a new strategy for characterization of gold nanorods (GNRs) that are of specific interest for biomedical applications due to their unique size-dependent longitudinal surface plasmon resonance band in the visible to near-infrared spectral region. More precisely, we characterized GNRs conjugated with short and long synthetic glycopolymers for biosensing of lectins in terms of particle size, coating thickness, and/or mobility properties in comparison with the bare GNRs. This endeavor requires a multidisciplinary approach including a new comprehensive set of fit-for-purpose analytical tools being high-resolution single-particle inductively coupled plasma-mass spectrometry (HR-spICP-MS) and electrical asymmetric-flow field-flow-fractionation hyphenated to a multi-angle light scattering detector (EAF4-MALS). GNRs were separated and characterized via EAF4-MALS in terms of their size and charge, while HR-spICP-MS provided information on the particle number density, size, size distribution, and the dimensional characterization. In addition, EAF4-MALS appears to be suitable for estimating coating thickness of glycoconjugated GNRs. Full article