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Keywords = glycotargeting

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14 pages, 4525 KB  
Article
A Novel Preparation Technique for Human Nasal Respiratory Mucosa to Disclose Its Glycosylation Pattern for Bioadhesive Drug Delivery
by Julia Clara Gausterer, Michael Schlager, Navid Ahmadi, Michael Nieratschker, Valerie Dahm, Michael Wirth, Christoph Arnoldner, Clemens Honeder and Franz Gabor
Pharmaceutics 2023, 15(3), 973; https://doi.org/10.3390/pharmaceutics15030973 - 17 Mar 2023
Cited by 1 | Viewed by 2591
Abstract
To shed some light on glycotargeting as a potential strategy for nasal drug delivery, a reliable preparation method for human nasal mucosa samples and a tool to investigate the carbohydrate building blocks of the glycocalyx of the respiratory epithelium are required. Applying a [...] Read more.
To shed some light on glycotargeting as a potential strategy for nasal drug delivery, a reliable preparation method for human nasal mucosa samples and a tool to investigate the carbohydrate building blocks of the glycocalyx of the respiratory epithelium are required. Applying a simple experimental setup in a 96-well plate format together with a panel of six fluorescein-labeled lectins with different carbohydrate specificities allowed for the detection and quantification of accessible carbohydrates in the mucosa. As confirmed by binding experiments at 4 °C, both quantitatively by fluorimetry and qualitatively by microscopy, the binding of wheat germ agglutinin exceeded that of the others by 150% on average, indicating a high content of N-acetyl-D-glucosamine and sialic acid. Providing energy by raising the temperature to 37 °C revealed uptake of the carbohydrate-bound lectin into the cell. Moreover, repeated washing steps during the assay gave a slight hint as to the influence of mucus renewal on bioadhesive drug delivery. All in all, the experimental setup reported here for the first time is not only a suitable approach to estimating the basics and potential of nasal lectin-mediated drug delivery but also meets the needs for answering a broad variety of scientific questions involving the use of ex vivo tissue samples. Full article
(This article belongs to the Section Drug Targeting and Design)
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34 pages, 10917 KB  
Review
Cyclodextrin-Based Functional Glyconanomaterials
by Gonzalo Rivero-Barbarroja, Juan Manuel Benito, Carmen Ortiz Mellet and José Manuel García Fernández
Nanomaterials 2020, 10(12), 2517; https://doi.org/10.3390/nano10122517 - 15 Dec 2020
Cited by 33 | Viewed by 6120
Abstract
Cyclodextrins (CDs) have long occupied a prominent position in most pharmaceutical laboratories as “off-the-shelve” tools to manipulate the pharmacokinetics of a broad range of active principles, due to their unique combination of biocompatibility and inclusion abilities. The development of precision chemical methods for [...] Read more.
Cyclodextrins (CDs) have long occupied a prominent position in most pharmaceutical laboratories as “off-the-shelve” tools to manipulate the pharmacokinetics of a broad range of active principles, due to their unique combination of biocompatibility and inclusion abilities. The development of precision chemical methods for their selective functionalization, in combination with “click” multiconjugation procedures, have further leveraged the nanoscaffold nature of these oligosaccharides, creating a direct link between the glyco and the nano worlds. CDs have greatly contributed to understand and exploit the interactions between multivalent glycodisplays and carbohydrate-binding proteins (lectins) and to improve the drug-loading and functional properties of nanomaterials through host–guest strategies. The whole range of capabilities can be enabled through self-assembly, template-assisted assembly or covalent connection of CD/glycan building blocks. This review discusses the advancements made in this field during the last decade and the amazing variety of functional glyconanomaterials empowered by the versatility of the CD component. Full article
(This article belongs to the Special Issue Functional Glyconanomaterials)
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18 pages, 831 KB  
Review
Legume Lectins: Proteins with Diverse Applications
by Irlanda Lagarda-Diaz, Ana Maria Guzman-Partida and Luz Vazquez-Moreno
Int. J. Mol. Sci. 2017, 18(6), 1242; https://doi.org/10.3390/ijms18061242 - 12 Jun 2017
Cited by 147 | Viewed by 18123
Abstract
Lectins are a diverse class of proteins distributed extensively in nature. Among these proteins; legume lectins display a variety of interesting features including antimicrobial; insecticidal and antitumor activities. Because lectins recognize and bind to specific glycoconjugates present on the surface of cells and [...] Read more.
Lectins are a diverse class of proteins distributed extensively in nature. Among these proteins; legume lectins display a variety of interesting features including antimicrobial; insecticidal and antitumor activities. Because lectins recognize and bind to specific glycoconjugates present on the surface of cells and intracellular structures; they can serve as potential target molecules for developing practical applications in the fields of food; agriculture; health and pharmaceutical research. This review presents the current knowledge of the main structural characteristics of legume lectins and the relationship of structure to the exhibited specificities; provides an overview of their particular antimicrobial; insecticidal and antitumor biological activities and describes possible applications based on the pattern of recognized glyco-targets. Full article
(This article belongs to the Special Issue Plant Lectins: From Model Species to Crop Plants)
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