Search Results (4)

Solid-state microbial fermentation (SSMF) has been established as an effective bioprocessing strategy to augment the nutritional value of plant-derived feed substrates while reducing anti-nutritional factors (ANFs). However, there have been limited studies on the effects of microbial solid-state fermentation on the nutritional value and potential functional components in cottonseed hulls. This study investigated the nutritional enhancement of cottonseed hulls through anaerobic solid-state fermentation mediated by Candida utilis CU-3, while exploring the functional potential of the fermented feed by analyzing fungal community dynamics and metabolite profiling. The laboratory-preserved free gossypol-degrading strain Candida utilis CU-3 was inoculated into unsterilized, crushed, and screened cottonseed hulls for solid-state fermentation at room temperature for 10 days. The results demonstrated that, compared to the control group, the experimental group achieved a 61.90% increase in free gossypol degradation rate, a 27.78% improvement in crude protein content, and a 5.07% reduction in crude fiber, while crude fat showed no significant difference. During the fermentation process, microbial diversity decreased, and Candida utilis CU-3 became the dominant species. Untargeted metabolomics data revealed that cottonseed hulls inoculated with Candida utilis CU-3 produced functional bioactive compounds during fermentation, including chrysin, myricetin (anti-inflammatory, antibacterial, and antioxidant activities), and ginsenoside Rh2 (anticancer, antibacterial, and neuroprotective properties). This study demonstrates that inoculating Candida utilis CU-3 into cottonseed hulls enhances their health-promoting potential through the biosynthesis of diverse functional metabolites, providing a theoretical foundation for improving the nutritional profile of cottonseed hull-fermented feed. Full article

Volvariella volvacea was grown on cottonseed hull waste compost and divided into high-yield (HBE) and low-yield (LBE) conditions. Gene sequencing was used to examine bacterial and fungal populations during cottonseed husk waste composting. At the end of fermentation, the dominant bacterial genera in the HBE compost were Chelatococcus and Thermobacillus, while Symbiobacterium and Acinetobacter were more abundant in the LBE compost. Ascomycota and Basidiomycota dominated all the composting phases. The Ascomycota genera Colletotrichum, Pichia, Mycothermus, and Thermomyces dominated in phase II of HBE composting. The LBE compost had higher abundances of the Basidiomycota genera Cystofilobasidium and Cryptococcus than the HBE compost. The predicted pathotroph and saprotroph-symbiotroph abundances were more positively linked to HBE composting phase II than to LBE composting. High-biological-efficiency microbial communities are characterized by high pH, carbon, and nitrogen levels. Changes in physiochemical traits, microbial diversity, and metabolism affect the V. volvacea yield. Full article

This investigation aimed to ascertain the efficacy of korshinsk peashrub as a viable substitute for cottonseed hull in the cultivation substrate of Agrocybe aegerita. The study incorporated korshinsk peashrub into the growth medium at incremental concentrations of 20%, 40%, and 60%, and subjected these blends to both fermentation and non-fermentation processes. Through rigorous assessment of yield of fruiting bodies, biological efficiency, nutrient profile, amino acid composition, and the integration of ecological and socio-economic advantages, an optimal substrate formulation was discerned. The findings revealed that the fermentation substrate FT2, with 40% korshinsk peashrub supplanting cottonseed hull, emerged as the superior blend following a comprehensive analysis. This formula notably yielded the highest crude protein and polysaccharide contents at 26.60% and 4.46%, respectively—an increase of 4.51% and 12.34% over the control. Consequently, these results suggest that korshinsk peashrub is a promising, cost-effective, and efficacious additive, capable of enhancing the yield and quality of A. aegerita and potentially replacing cottonseed hull extensively. Full article

To explore whether or not the gossypol varied in cottonseed by-products affect rumen degradability and fermentation efficiency, an in vitro cumulative gas production experiment was applied with mixed rumen microorganism to compare rumen fermentation characteristics of whole cottonseed (WCS, n = 3 samples), cottonseed meal (CSM, n = 3 samples), and cottonseed hull (CSH, n = 2 samples). The five-replicate fermentation per sample per incubation time continuously lasted for 0.5, 1.5, 3, 6, 12, 24, 36, and 48 h with an automated gas production recording system. Regardless of distinct nutrient differences, the free gossypol level in these cottonseed by-products ranked: WCS > CSH > CSM. After 48 h of incubation, the in vitro dry matter degradability and ammonia N concentration ranked as: CSM > WCS > CSH. The cumulative gas production and total volatile fatty acid (VFA) levels in the culture fluids ranked: CSM > CSH > WCS, in which the average production rate ranked: CSM > WCS > CSH. Regarding the molar VFA pattern, WCS in comparison with CSH and CSM presented the lowest production of non-glucogenic acids (e.g., acetate) and exhibited the highest fermentation efficiency of energy from carbohydrates to VFAs. There was a significant negative correlation between the gossypol content and cumulative gas and total VFA production, suggesting that the greater gossypol in cottonseed by-products, the more detrimental effect occurred for rumen fermentation. In a brief, WCS exhibited mediocre rumen degradability and less microbial fermentation efficiency than CSH and CSM, depending on their gossypol levels. Full article