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Keywords = elctrospun nanofibers

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12 pages, 9768 KiB  
Article
ECM Decorated Electrospun Nanofiber for Improving Bone Tissue Regeneration
by Yong Fu, Lili Liu, Ruoyu Cheng and Wenguo Cui
Polymers 2018, 10(3), 272; https://doi.org/10.3390/polym10030272 - 6 Mar 2018
Cited by 37 | Viewed by 7012
Abstract
Optimization of nanofiber surface properties can lead to enhanced tissue regeneration outcomes in the context of bone tissue engineering. Herein, we developed a facile strategy to decorate elctrospun nanofibers using extracellular matrix (ECM) in order to improve their performance for bone tissue engineering. [...] Read more.
Optimization of nanofiber surface properties can lead to enhanced tissue regeneration outcomes in the context of bone tissue engineering. Herein, we developed a facile strategy to decorate elctrospun nanofibers using extracellular matrix (ECM) in order to improve their performance for bone tissue engineering. Electrospun PLLA nanofibers (PLLA NF) were seeded with MC3T3-E1 cells and allowed to grow for two weeks in order to harvest a layer of ECM on nanofiber surface. After decellularization, we found that ECM was successfully preserved on nanofiber surface while maintaining the nanostructure of electrospun fibers. ECM decorated on PLLA NF is biologically active, as evidenced by its ability to enhance mouse bone marrow stromal cells (mBMSCs) adhesion, support cell proliferation and promote early stage osteogenic differentiation of mBMSCs. Compared to PLLA NF without ECM, mBMSCs grown on ECM/PLLA NF exhibited a healthier morphology, faster proliferation profile, and more robust osteogenic differentiation. Therefore, our study suggests that ECM decoration on electrospun nanofibers could serve as an efficient approach to improving their performance for bone tissue engineering. Full article
(This article belongs to the Special Issue Polymer Scaffolds for Biomedical Application)
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