Search Results (4)

Antioxidants protect cellular function and structure by neutralizing the oxidative stress caused by increased reactive oxygen species (ROS) during sperm freezing. Studies on cryopreservation using various antioxidants have demonstrated encouraging results. Many studies have used antioxidants to increase the efficiency of sperm freezing and to improve the success rate of artificial insemination and pregnancy. Manganese (III) tetrakis (4-benzoic acid) porphyrin chloride (MnTBAP) is a newly synthesized antioxidant with positive effects on sperm morphology and capacitation in humans, rams, and stallions. In this study, porcine semen was treated with 0, 50, 100, and 150 μM of MnTBAP based on a Tris–egg-yolk extender and frozen to determine whether MnTBAP can assist the status of sperm during cryopreservation. First, motility was assessed using the computer-assisted sperm analysis (CASA) system, with the 100 μM treatment group showing the highest motile rate (66.8%) compared with that of the other groups (control, 51.1%; 50 μM and 150 μM, 59.6%); therefore, the remaining analyses were conducted comparing the two groups (control vs. 100 μM group; p < 0.01). Second, fluorescence staining was applied to examine the control and 100 μM groups using fluorescence microscopy. The viability (41.7% vs. 62.4%) and the acrosome integrity (77.9% vs. 86.4%) differed significantly (p < 0.05). In addition, the mitochondrial membrane potential (MMP) was 46.5% vs. 51.9%; the fragmentation rate, estimated using the Sperm-sus-Halomax kit, was 63.4% vs. 57.4%; and the detected caspase activity was 30.1% vs. 22.9%. These tended to be higher in the treated group but did not differ significantly. Third, measurements using FACSLyric revealed that the 100 μM treatment group exhibited a state of elevated normal lipid arrangement within the plasma membrane and diminished levels of apoptosis and ROS (p < 0.01). We assessed the expression of genes relevant to antioxidant effectiveness using real-time RT-qPCR. Our findings indicated significant alterations in the expression levels of various mRNA species, with the exception of NOX5 (p < 0.05). Finally, the straws were dissolved and used to treat matured denuded oocytes to investigate the effect on fertilization and embryo development in vitro. The cleavage rate was (77.6% vs. 84.1%), and the blastocyst rate was 9.7% vs. 11.4% (p < 0.05). In conclusion, these results suggest that MnTBAP positively affected sperm freeze–thawing, improving the fertilization capacity, and leading to increased embryo development. Full article

High-resolution surface freeze/thaw (F/T) information is valuable for hydrological, frost creep and gelifluction/solifluction, and climate prediction studies. Currently, large-scale, high-resolution F/T detection is restricted by low spatial resolution of passive microwave remote sensing sensors or low temporal resolution of synthetic aperture radar (SAR) data. In this study, we propose a new method for detecting daily land surface F/T state at 1 km spatial resolution by combining the Sentinel-1 radar and the Advanced Microwave Scanning Radiometer 2 (AMSR2) with leaf area index (LAI) data. A non-linear relationship is established between the 1 km F/T index from Sentinel-1 with 1 km F/T index from AMSR2 (FTI) and 1 km LAI data. The 1 km FTI is a disaggregation of the 25 km FTI obtained from AMSR2. This non-linear relationship is then applied to daily 1 km FTI and LAI data to predict the 1 km daily F/T index, based on which the F/T status is detected with grid-cell-based F/T thresholds. The overall accuracy of this daily 1 km F/T is more than 88.1% when evaluated with the in situ 5 cm soil temperature over China and Canada. This study is valuable for detecting daily, high-resolution F/T status and is helpful for studies related to disaster and climate prediction. Full article

With global warming, permafrost is undergoing degradation, which may cause thawing subsidence, collapse, and emission of greenhouse gases preserved in previously frozen permafrost, change the local hydrology and ecology system, and threaten infrastructure and indigenous communities. The Qinghai-Tibet Plateau (QTP) is the world’s largest permafrost region in the middle and low latitudes. Permafrost status monitoring in the QTP is of great significance to global change and local economic development. In this study, we used 66 scenes of ALOS data (2007–2009), 73 scenes of ALOS-2 data (2015–2020) and 284 scenes of Sentinel-1 data (2017–2021) to evaluate the spatial and temporal permafrost deformation over the 83,000 km² in the northern QTP, passing through the Tuotuohe, Beiluhe, Wudaoliang and Xidatan regions. We use the SBAS-InSAR method and present a coherence weighted least squares estimator without any hypothetical model to calculate long-term deformation velocity (LTDV) and maximum seasonal deformation (MSD) without any prior knowledge. Analysis of the ALOS results shows that the LTDV ranged from −20 to +20 mm/year during 2007–2009. For the ALOS-2 and Sentinel-1 results, the LTDV ranged from −30 to 30 mm/year during 2015–2021. Further study shows that the expansion areas of permafrost subsidence are concentrated on braided stream plains and thermokarst lakes. In these areas, due to glacial erosion, surface runoff and river alluvium, the contents of water and ground ice are sufficient, which could accelerate permafrost subsidence. In addition, by analyzing LTDV and MSD for the different periods, we found that the L-band ALOS-2 is more sensitive to the thermal collapse of permafrost than the C-band sensor and the detected collapse areas (LTDV < −10 mm/year) are consistent with the GF-1/2 thermal collapse dataset. This research indicates that the InSAR technique could be crucial for monitoring the evolution of permafrost and freeze-thaw disasters. Full article

Transition of freeze/thaw (F/T) affects land-atmospheric interactions and other biospheric dynamics. Global F/T statuses are normally monitored using microwave remote sensing, but at coarse resolutions (e.g., 25 km). Integration of coarse microwave remote sensing data with finer satellite products represents an opportunity to further enhance our ability to map F/T statuses regionally and globally. Here, we implemented and tested an approach to generate daily F/T status maps at a 5-km spatial resolution through the fusion of passive microwave data from AMSR2 and land surface temperature products from MODIS, using China as our study area for the year 2013 and 2014. Moreover, possible influences from elevation, vegetation, seasonality, etc., were also analyzed, as such analysis provides a direction to improve the approach. Overall, our freeze/thaw maps agreed well with ground reference observations, with an accuracy of ~86.6%. The new F/T maps helped to identify regions subject to frequent F/T transitions through the year, such as the Qinghai-Tibetan Plateau, Xinjiang, Gansu, Heilongjiang, Jilin, and Liaoning Province. This study indicates that the combination of AMSR2 and MODIS observations provides an effective method to obtain finer F/T maps (5-km or lower) for extensive regions. The finer F/T maps improve our knowledge of the F/T state detected by satellite remote sensing, and have a wide range of applications in regional studies considering land surface heterogeneity and models (e.g., community land models). Full article