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Keywords = cellobionic acid

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15 pages, 2884 KB  
Article
Integration of Physiological Analysis and Untargeted Metabolomics to Explore Differences in Quality Among Four Sweet Cherry Cultivars
by Guoqin Li, Xiaosa Yang, Zhonghua Cao, Fei Li, Guifeng Li and Erihemu
Foods 2025, 14(18), 3207; https://doi.org/10.3390/foods14183207 - 15 Sep 2025
Viewed by 555
Abstract
This study compared the quality parameters of four popular sweet cherry fruits (“Tieton”, “Pioneer”, “Sunburst”, and “Huangmi”) in Shanxi Province and used untargeted metabolomics to analyze the differential metabolites (DMEs) among them. The results showed that the four fruits have distinct differences in [...] Read more.
This study compared the quality parameters of four popular sweet cherry fruits (“Tieton”, “Pioneer”, “Sunburst”, and “Huangmi”) in Shanxi Province and used untargeted metabolomics to analyze the differential metabolites (DMEs) among them. The results showed that the four fruits have distinct differences in their skin color, texture, size, weight, and solid-to-acid ratio. Notably, “Huangmi” fruit showed greater physical damage and bitterness and lower overall likeability than the other three fruits after short-distance road transportation. Untargeted metabolomics identified 97 DMEs among the four fruits. Specifically, the levels of 44 DMEs (such as cellobionate, allose, L-histidine, kaempferol, ascorbic acid, cinnamaldehyde, and Qing Hau Sau), 22 DMEs (such as raffinose, neochlorogenic acid, epicatechin, carvone, and (S)-norcoclaurine), 9 DMEs (such as melibiitol, 3′-ketolactose, and all-trans-retinoic acid), and 3 DMEs (D-maltose, shikimic acid, and selenocysteine) were highest in the “Huangmi”, “Sunburst”, “Pioneer”, and “Tieton” fruits, respectively. Moreover, the red cultivars (“Tieton”, “Pioneer”, and “Sunburst”) showed a higher citrulline content than the yellow cultivar (“Huangmi”). This study can serve as a reference for cultivar breeding, market segmentation, growers, and related industries, laying a foundation for further research on food nutrition and human health. Full article
(This article belongs to the Section Food Quality and Safety)
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14 pages, 2681 KB  
Article
Efficient Bio-Oxidation of Cellobiose with Engineered Gluconobacter oxydans to Provide Highly Concentrated Cellobionic Acid
by Emmeran Bieringer, Lisa Pütthoff, Arne Zimmermann, Mariana de Souza Góes, Uraz Yilmaz, Armin Ehrenreich, Wolfgang Liebl and Dirk Weuster-Botz
Processes 2024, 12(7), 1464; https://doi.org/10.3390/pr12071464 - 13 Jul 2024
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Abstract
Cellobionic acid (CBA) can be obtained through the oxidation of cellobiose, the monomer of cellulose. CBA serves as a plant-based alternative to its stereoisomer lactobionic acid, which is used in the pharmaceutical, cosmetic, and food industries. Gluconobacter oxydans is a well-established whole-cell biocatalyst [...] Read more.
Cellobionic acid (CBA) can be obtained through the oxidation of cellobiose, the monomer of cellulose. CBA serves as a plant-based alternative to its stereoisomer lactobionic acid, which is used in the pharmaceutical, cosmetic, and food industries. Gluconobacter oxydans is a well-established whole-cell biocatalyst with membrane-bound dehydrogenases (mDH) for regio-specific oxidations. As G. oxydans wildtype cells show low cellobiose oxidation activities, the glucose mDH from Pseudomonas taetrolens was overexpressed in G. oxydans BP9, a multi mDH deletion strain. Whole-cell biotransformation studies were performed with resting cells of the engineered G. oxydans in stirred tank bioreactors. Initial biomass specific cellobionate formation rates increased with increasing cellobiose concentrations up to 190 g L−1, and were constant until the solubility limit. The maximal volumetric CBA formation rates and the oxygen uptake rates increased linearly with the concentration of engineered G. oxydans. This enables the estimation of the maximum biocatalyst concentration limited by the maximum oxygen transfer rate of any bioreactor. Thus, 5.2 g L−1 G. oxydans was sufficient to produce 502 g L−1 CBA with >99% yield in a simple aerobic batch process. The highly concentrated CBA will reduce downstream processing costs considerably after cell separation. Full article
(This article belongs to the Special Issue Development, Modelling and Simulation of Biocatalytic Processes)
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