Search Results (7)

The aim of this study was to examine relationships among serum progestagens, cortisol, and prolactin in pregnant and normal cycling Asian elephants living in tourist camps in northern Thailand. Samples were collected twice a month for 22 months from nine elephants. Of those, four were pregnant (24.3 ± 2.9 years of age; range 21–28 years) and five (20.2 ± 9.6 years; range 8–34 years) exhibited normal ovarian cycles based on serum progestagen analyses. Gestation was divided into three periods: 1st (week 1–31), 2nd (week 32–62), and 3rd (week 63 to parturition), while the estrous cycle was divided into the follicular and luteal phases. Serum progestagens were higher during the luteal phase of the cycle (p < 0.003), whereas cortisol and prolactin were similar. In pregnant elephants, there were no differences in serum progestagens or cortisol concentrations across the three gestational periods, whereas prolactin concentrations increased significantly during the 2nd and 3rd periods (p < 0.0001). By contrast, prolactin concentrations in nonpregnant elephants were consistently low throughout the ovarian cycle. In one cycling female, prolactin concentrations were similar to pregnant elephants, perhaps because she was an allomother to two calves. Another cycling female exhibited consistently elevated cortisol concentrations, 5 to 10 times higher than the other elephants. There were no correlations between serum progestagens, cortisol, and prolactin throughout gestation; however, serum progestagens and cortisol were positively related in cycling elephants (r = 0.386, p < 0.001). From our results, there were a number of individual differences in reproductive hormonal patterns, so it is important to develop personalized monitoring programs for each elephant to enhance breeding success and create sustaining captive populations of elephants in Asia. Full article

To minimize false-positive cases in newborn screening by tandem mass spectrometry in Japan, practical second-tier liquid chromatography-tandem mass spectrometry analyses have been developed using a multimode ODS column with a single set of mobile phases and different gradient elution programs specific to the analysis of acylcarnitines, acylglycines, amino acids, and organic acids. Most analyses were performed using underivatized samples, except for analysis of methylcitric acid, and careful conditioning of the column was necessary for analyses of organic acids. Our second-tier tests enabled us to measure many metabolites useful for detection of target disorders, including allo-isoleucine, homocysteine, methylmalonic acid, and methylcitric acid. We found that accumulation of 3-hydroxyglutaric acid was specific to glutaric acidemia type I and that the ratio of 3-hydroxyisovaleric acid to 3-hydroxyisovalerylcarnitine was useful to detect newborns of mothers with 3-methylcrotonyl-CoA carboxylase deficiency. Data from the analysis of short-chain acylcarnitine and acylglycine were useful for differential diagnosis in cases positive for C5-OH-acylcarnitine or C5-acylcarnitine. Full article

In buffaloes and other mammalian farm species, the mother provides food and protection to the young, but she is also the main source of behavioral and social learning for the offspring. It is important that mother and young establish a bond based on a learning mechanism defined as “imprinting” early after parturition during the sensitive period, on which the welfare and survival of the offspring will depend. This review aims to summarize and discuss current knowledge regarding the imprinting process, the neurobiological pathways that are triggered during this sensitive period, and the development of the cow–calf bond. Touch, hearing, vision, and smell seem to be the predominant senses involved during imprinting in buffaloes and other mammalian farm species. In buffalo, bonding is very particular due to the expression of specific behaviors, such as allo-suckling and communal rearing. In general, imprinting and the subsequent bond may be affected by the lack of experience of the mothers or dystocic parturitions, which occur most frequently with male calves and in primiparous dams. The main problems in the development of this process include lack of seeking a protected and isolated place to give birth; moving from the birth-site after parturition; insufficient postpartum care; aversion or aggressiveness towards the newborn, or abandonment of the newborn. The process can develop differently according to the species. However, the correct development of the cow–calf relationship represents, regardless of the species, a key factor for their fitness. Full article

In high-income, low-fertility (HILF) settings, the mother’s partner is a key provider of childcare. However, it is not clear how mothers without partners draw on other sources of support to raise children. This paper reports the findings from a survey of 1532 women in the United Kingdom and the United States, in which women described who provided childcare for a focal child and how frequently they did so. We use multivariate Bayesian regression models to explore the drivers of support from partners, maternal kin, and other allomothers, as well as the potential impact of allomothering on women’s fertility. Relative to mothers who are in a stable first marriage or cohabitation, mothers who are unpartnered rely more heavily on fewer maternal kin, use more paid help, and have networks which include more non-kin helpers. Repartnered mothers received less help from their partners in the UK and less help from maternal kin in both countries, which US mothers compensated for by relying on other helpers. While repartnered mothers had higher age-adjusted fertility than women in a first partnership, allomaternal support was not clearly related to the mother’s fertility. These findings demonstrate the importance of partners but also of allomothering more broadly in HILF settings. Full article

Orangutans depend on social learning for the acquisition of survival skills. The development of skills is not usually assessed in rescued orphans’ pre-release. We collected data of seven orphans over an 18-months-period to monitor the progress of ontogenetic changes. The orphans, 1.5–9 years old, were immersed in a natural forest environment with human surrogate mothers and other orphans. Social interactions deviated significantly from those of wild mother-reared immatures. Infants spent more time playing socially with peers, at the expense of resting and solitary play. Infants were also more often and at an earlier age distant from their human surrogate mothers than wild immatures are from their biological mothers. We found important changes towards an orangutan-typical lifestyle in 4- to 7-year-old orphans, corresponding to the weaning age in maternally reared immatures. The older orphans spent less time interacting with human surrogate mothers or peers, started to use the canopy more than lower forest strata and began to sleep in nests in the forest. Their time budgets resembled those of wild adults. In conclusion, juvenile orphans can develop capacities that qualify them as candidates for release back into natural habitat when protected from humanising influences and immersed in a species-typical environment. Full article

Fetal/neonatal allo-immune thrombocytopenia (FNAIT) results from maternal immunization against fetal platelet-specific antigens (HPA) inherited from the father. Most cases involve HPA located on glycoproteins (GP) IbIX, IaIIa and IIbIIIa. Iso-immunizations can also occur in the absence of expression of membrane proteins, such as GPIIb or GPIIIa in Glanzmann patients. CD36 (also called glycoprotein GPIV) deficiency is observed in 3 to 5% of Asian and African populations. We report here the case of a 41-year-old Canadian woman originated from Africa, who delivered a male dead new-born at 39 weeks of gestation. A massive intracranial haemorrhage was identified as being the obvious cause of death. No platelet antibody against GPIbIX, IaIIa, and IIbIIIa was identified by the gold-standard Monoclonal Antibody-specific Immobilization of Platelet Antigens (MAIPA) assay. Surprisingly, anti CD36 iso-antibodies were identified in the maternal serum with a new bead-based multiplex assay. The CD36 gene was sequenced for both parents, and a mutation was identified on Exon 10 of the mother’s CD36 gene, which was absent for the father: NM_000072.3:c.975T>G inducing a STOP codon at position 325 of the mature protein. The absence of CD36 expression on the mother’s platelets was confirmed by flow cytometry. Full article

Alloimmunisation to platelet antigens is not uncommon; a large number of females, having had pregnancies, developed antibodies to Human Leukocyte Antigen (HLA) moieties harboured on their foetuses’ cells (inherited from the father(s)) that may conflict with further pregnancies and transfused Platelet Components occasionally. This is possible since platelets constitutionally express HLA class I molecules (though in copy numbers that consistently differ among individuals). Platelets also express HPA moieties that are variants of naturally expressed adhesion and aggregation molecules; HPA differences between mothers and foetuses and between donors and recipients explain alloimmune conflicts and consequences. Lastly, platelets express ABO blood group antigens, which are rarely immunising, however transfusion mismatches in ABO groups seem to be related to immunisation in other blood and tissue groups. Transfusion also brings residual leukocytes that may also immunise through their copious copy numbers of HLA class I (rarely class II on activated T lymphocytes, B cells, and dendritic cells). In addition, residual red blood cells in platelet concentrates may induce anti-red blood cell allo-antibodies. This short review aims to present the main mechanisms that are commonly reported in alloimmunisation. It also critically endeavours to examine paths to either dampen alloimmunisation occurrences or to prevent them. Full article