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Keywords = acylanilide fungicide

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12 pages, 2450 KiB  
Article
Metalaxyl Degradation by Mucorales Strains Gongronella sp. and Rhizopus oryzae
by Maria Rosário Martins, Cledir Santos, Pablo Pereira, Júlio Cruz-Morais and Nelson Lima
Molecules 2017, 22(12), 2225; https://doi.org/10.3390/molecules22122225 - 14 Dec 2017
Cited by 12 | Viewed by 4799
Abstract
In this study, the degradation of metalaxyl was investigated in the presence of two Mucorales strains, previously isolated from soil subjected to repeated treatments with this fungicide and selected after enrichment technique. Fungal strains were characterised by a polyphasic approach using phylogenetic analysis [...] Read more.
In this study, the degradation of metalaxyl was investigated in the presence of two Mucorales strains, previously isolated from soil subjected to repeated treatments with this fungicide and selected after enrichment technique. Fungal strains were characterised by a polyphasic approach using phylogenetic analysis of the Internal Transcribed Spacer (ITS) gene region, phenotypic characterisation by Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) spectral analysis, and growth kinetics experiments. The strains were identified as Gongronella sp. and Rhizopus oryzae. The fungal growth kinetics in liquid cultures containing metalaxyl fits with Haldane model. Under laboratory conditions, the ability of Gongronella sp. and R. oryzae cultures to degrade metalaxyl was evaluated in liquid cultures and soil experiments. Both species were able to: (a) use metalaxyl as the main carbon and energy source; and (b) degrade metalaxyl in polluted soils, with rates around 1.0 mg kg1 d1. This suggests these strains could degrade metalaxyl in soils contaminated with this fungicide. Full article
(This article belongs to the Section Chemical Biology)
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